We narrowed to 2,477 results for: T2A

1. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

   Plasmid

   #171802

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-tetO-hOKMSOX17FNV

   Plasmid

   #206382

   Purpose

   Expresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   OCT4, KLF4, c-MYC, SOX17FNV (POU5F1, KLF4, MYC, SOX17 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6-racRNA-B

   Plasmid

   #200825

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-U6-racRNA-C

   Plasmid

   #200826

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-U6-racRNA-D

   Plasmid

   #200827

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

   Plasmid

   #171813

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-Ova-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GT-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

   Plasmid

   #241396

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-Ngn2-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3, NEUROG2 Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE; CAG

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid

    #171817

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPaint-mScarlet-F-hgp100-BlastR [M1G]

    Plasmid

    #171815

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-hgp100-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

    Plasmid

    #171811

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid

    #171810

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

    Plasmid

    #171808

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-F-Ova-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPaint-mNeon-F-hgp100-BlastR [M1G]

    Plasmid

    #171806

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-F-hgp100-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid

    #171804

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge