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Showing: 3451 - 3480 of 164506 results

  8. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid
    #239102
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    POU4F2 (POU4F2 Human)
    Use
    Donor plasmid for integration into the human geno…
    Tags
    p2A-tdTomato-p2A-Thy1.2
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous POU4F2
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239102-plasmid-map-sequence-id-474475

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  13. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222853
    Purpose
    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222853-plasmid-map-sequence-id-445015

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  14. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222855
    Purpose
    Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222855-plasmid-map-sequence-id-445010

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  21. qTAG-N-Blast-mNeon-ACTB

    Plasmid
    #207751
    Purpose
    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207751-plasmid-map-sequence-id-416864

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  22. TFORF2370

    Plasmid
    #142056
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MBD4 (MBD4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142056-plasmid-map-sequence-id-395678

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  27. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid
    #170236
    Purpose
    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CHUK (CHUK Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    S176E, S180E
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170236-plasmid-map-sequence-id-334484

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  28. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  29. RCP83

    Plasmid
    #163466
    Purpose
    Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP
    Depositor
    Frank Albert
    Article
    Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1
    Inserts
    Promoter fragment (Sharon2012_1922)
    His3 minimal promoter
    yeGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163466-plasmid-map-sequence-id-322582

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Showing: 3451 - 3480 of 164506 results