We narrowed to 81,725 results for: MYC;
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Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSum-LacZ-gfp
Plasmid#109285PurposeExpresses gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationGFP modified for expression in prokaryotes; inclu…PromoterLacZalphaAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC2_10_dCas9_VPR_HC_sgRNA
Plasmid#163712PurposeYeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD100
Plasmid#212924PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKCcas9dO
Plasmid#62552PurposeHigh efficiency Streptomyces genome editing by CRISPR/Cas9 systemDepositorInsertsScocas9
sgRNA ()
act-orf4 homology arm
UseCRISPR; Bacterial genome editingExpressionBacterialPromoterj23119 and ptipAAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GFP
Plasmid#39289DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Mutation-1163 to +6 of nmt1 locusAvailable SinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMV-LEU2
Plasmid#65334Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUsDepositorInsertLEU2
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1
Plasmid#41607DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
PTEF1-yEGFPCLN2PEST-pRS406
Plasmid#64406PurposeTEF promoter driven GFP expressionDepositorInsertTEF1 promoter (TEF1 Budding Yeast)
TagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastPromoterTEF1 promoterAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Level 1 P1 OsActinP:Hygint:NosT
Plasmid#165423PurposeGoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassetteInsertOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
UseSynthetic Biology; L1 p1(r) golden gate (moclo) h…PromoterOs Actin promoter :: Hygromycin resistance gene (…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a
Plasmid#158713PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinumDepositorInsertthe MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
ExpressionPlantPromoterATUBI10 and Medtr8g059790(KPI)Available SinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZJOM30
Plasmid#133673PurposeIntegration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR
Plasmid#24585DepositorInsertpADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdBIP1proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
Plasmid#83947PurposegRNA cassette-carrying vector with natMX markerDepositorInsertgRNA targeting ADE2 gene
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC2_3_dCas9_Mxi1_sgRNA
Plasmid#163708PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressorDepositorInsertsdCas9
Mxi1+SV40 NLS
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR
Plasmid#24378DepositorInsertpADH1 yeCherry::p150yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM112B
Plasmid#210602PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
TagsmClover3ExpressionBacterial and YeastPromoterSpH2A/Bpro (divergent)Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-kanMX6-P3nmt1
Plasmid#39280DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Mutation-1163 to +6 of nmt1 locusAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-3HA
Plasmid#41608DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdH3proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZA26
Plasmid#158504PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM10
Plasmid#133639PurposeIntegration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM5
Plasmid#133635PurposeIntegration of CHS5-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1
Plasmid#41605DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-PURO
Plasmid#58246PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selectionDepositorInsertPuro(R)
UseLentiviral and RNAi; Gateway destionation vectorTagsTetR-NLS-T2AExpressionMammalianPromoterSFFVAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM22-Halo-FRB
Plasmid#197059PurposeExpression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
TagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ172
Plasmid#98409PurposeDonor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsupstream homologous region to delete lys9 in genome by recombination
downstream homologous region to delete lys9 in genome by recombination
UseCRISPR and Synthetic BiologyAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBC155
Plasmid#202274PurposeLevel 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent markerDepositorInsertbc182
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3HA
Plasmid#39283DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…Mutation-1163 to +6 of nmt1 locusAvailable SinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSF1C-A-RFP
Plasmid#172431PurposeStrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNoneExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
TagsFirst 8 codons of COX3MutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GST
Plasmid#41611DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMV-HIS3
Plasmid#65333Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUsDepositorInsertHIS3
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
ExpressionBacterialAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGCNK58
Plasmid#202461PurposePlasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cellsDepositorInsertResidues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
ExpressionBacterialAvailable SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only