We narrowed to 58,402 results for: cloning

1. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

   Plasmid

   #228363

   Purpose

   mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

   Plasmid

   #228362

   Purpose

   mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Rfxcas13d grna expression …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET MBP GFP LIC cloning vector (MBP-msfGFP)

   Plasmid

   #29750

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

   Plasmid

   #29725

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET MBP mCherry LIC cloning vector (MBP-mCherry)

   Plasmid

   #29747

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)

   Plasmid

   #166835

   Purpose

   Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

   Plasmid

   #30116

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 MBP prescission LIC cloning vector (HMPKS)

   Plasmid

   #29721

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and Prescission cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

    Plasmid

    #29654

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 MBP N10 TEV LIC cloning vector (2C-T)

    Plasmid

    #29706

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

    Plasmid

    #48322

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET His6 Gamma crystallin TEV LIC cloning vector (1X)

    Plasmid

    #29661

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, Gamma-crystallin, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 StrepII TEV LIC cloning vector (2HR-T)

    Plasmid

    #29718

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, Strep Ii, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

    Plasmid

    #48283

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

    Plasmid

    #29723

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mO…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)

    Plasmid

    #29724

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His6 Sumo TEV co-transformation cloning vector (13K-S)

    Plasmid

    #48321

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid

    #86933

    Purpose

    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

    Plasmid

    #30184

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge