We narrowed to 944 results for: trna
-
Plasmid#31174DepositorInsertMature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
TagsHis and thrombin cleavageExpressionBacterialAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH652-RLuc/maxFLuc
Plasmid#29698DepositorInsertFirefly Luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…Available SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
TrmD_HaeIn/pET28b(+) N,C-tag
Plasmid#12665DepositorInserttRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
TagsHisExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTH651-RLuc/minFLuc
Plasmid#29697DepositorInsertFirefly Luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…Available SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH490-tQSRTL
Plasmid#29699DepositorInserttR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
ExpressionYeastAvailable SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
TagsV5-His6ExpressionMammalianPromoterCMV and U6/H1Available SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
ExpressionBacterialPromoterGlnS (constitutive)Available SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialPromoteraraC and lppAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP WT
Plasmid#197567PurposeExpression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)
TagsV5-His6ExpressionMammalianPromoterCMV and U6/H1Available SinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-CMV-mCherry-1xU6-LeuIGI1
Plasmid#217367PurposeExpresses E. coli leucine tRNA variant "LeuIGI1" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli leucine tRNA mutant, "LeuIGI1" for TAG suppression
UseAAVExpressionMammalianMutationG6U, C78G, U83CPromoterU6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2
Plasmid#217368PurposeExpresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
UseAAVExpressionMammalianMutationC2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83CPromoterU6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32G, E107P, D158T, I159SAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtLeuRS
Plasmid#31079DepositorInsertMature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
TagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …Available SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-IodoY
Plasmid#113645PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.DepositorInsertIodoYRS and tRNA gene
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEVOL-MmAcKRS1-PylTUUA
Plasmid#182537PurposeN‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppressionDepositorInsertPylTUUA
ExpressionBacterialPromoterProKAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only