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We narrowed to 944 results for: trna

Showing: 221 - 240 of 944 results
  1. pDule2 - Mj Acd A9

    Plasmid
    #197652
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pDule- Mj Acd A9

    Plasmid
    #197651
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pAcBac1-Ma-sfGFP WT

    Plasmid
    #197567
    Purpose
    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    Tags
    V5-His6
    Expression
    Mammalian
    Promoter
    CMV and U6/H1
    Available Since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid
    #217368
    Purpose
    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV
    Insert
    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    Use
    AAV
    Expression
    Mammalian
    Mutation
    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C
    Promoter
    U6
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
  8. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
  9. PylRS-AS

    Plasmid
    #137908
    Purpose
    For incorporation of fluorophenylalanine derivatives
    Depositor
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    Containing N346A, C348S mutations
    Promoter
    pBAD
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 221 - 240 of 944 results