168,897 results

1. FLAG-SFPQ

   Plasmid

   #166960

   Purpose

   Expresses FLAG-tagged SFPQ protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)

   Viral Prep

   #105545-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   HI

   Tags

   EGFP

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52 and pTDH3

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRESpuroN3xFLAG-UFSP2

   Plasmid

   #204553

   Purpose

   Expresses 3xFLAG-tagged UFSP2 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFSP2 (UFSP2 Human)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-Cas9-2XNES-2ERT2

   Plasmid

   #120551

   Purpose

   Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

   Depositor

   Yu Wang

   Article

   Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

   Insert

   Cas9, NES and ERT2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-ERK1

   Plasmid

   #14747

   Depositor

   Rony Seger

   Article

   Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

   Insert

   ERK1 (MAPK3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaMKAR

   Plasmid

   #205315

   Purpose

   Fluorescent Reporter for CaMKII Activity

   Depositor

   Mark Anderson, Elizabeth Luczak

   Article

   Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

   Insert

   CaMKAR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFCIV-CEACAM6

    Plasmid

    #149303

    Purpose

    lentiviral packaging vector for CEACAM6 expression.

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    ubiquitin C

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ131B2.0

    Plasmid

    #99885

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LBR-mCherry

    Plasmid

    #172446

    Purpose

    Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    lamin B receptor (LBR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-TMPRSS2-blast

    Plasmid

    #158458

    Purpose

    Constitutive lentiviral expression of human TMPRSS2.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ142

    Plasmid

    #69294

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-CLIP170-C-18

    Plasmid

    #54043

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid

    #192453

    Purpose

    intronized ttLbCas12a for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Mutation

    temperature-tolerant mutation (D156R)

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid

    #135281

    Purpose

    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-CDK12 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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