We narrowed to 7,041 results for: Ank

1. -962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

   Plasmid

   #32735

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 Promoter (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

   Promoter

   -962 CCND1

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pRRG260-Cyp1A1

   Plasmid

   #99081

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Cyp1A1 (also known as h.49.4f)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   Cyp1A1 (also known as h.49.4f) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DCT.VV.Orf234

   Plasmid

   #12922

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf234
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

4. DCT.VV.Orf226

   Plasmid

   #12914

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf226
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

   Plasmid

   #52540

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   PhiC31o integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DCT.VV.Orf110

   Plasmid

   #12798

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf110
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52542

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   PhiC31o integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DCT.VV.Orf67

   Plasmid

   #12755

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf67
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

9. DCT.VV.Orf148

   Plasmid

   #12836

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf148
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

10. DCT.VV.Orf72

    Plasmid

    #12760

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    A34R
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

11. DCT.VV.Orf263

    Plasmid

    #12951

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf263
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pAG228 TNKS2 3'UTR mut

    Plasmid

    #12031

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    TNKS2 3'UTR mut (TNKS2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DCT.VV.Orf82

    Plasmid

    #12770

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf82
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

14. DCT.VV.Orf152

    Plasmid

    #12840

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf152
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-rev(CMV-TagBFP2)-EFS-mCherry-133-MRE

    Plasmid

    #235157

    Purpose

    Expresses the mCherry-based sensor for miR-133. TagBFP2 is expressed as a transduction reporter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    mCherry with a miR-133 MRE from Ankrd28
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE2-TetO-AncHolozoan Sox

    Plasmid

    #231528

    Purpose

    Expresses reconstructed Ancestral Holozoan Sox in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Ancestral Holozoan Sox HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE2-TetO-AncMetazoan Sox

    Plasmid

    #231527

    Purpose

    Expresses reconstructed Ancestral Metazoan Sox in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Ancestral Metazoan Sox HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE2-TetO-AncSoxCEF

    Plasmid

    #231526

    Purpose

    Expresses reconstructed Ancestral SoxCEF in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Ancestral SoxCEF HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-TetO-AncSoxB

    Plasmid

    #231524

    Purpose

    Expresses reconstructed Ancestral SoxB in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Ancestral SoxB HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2-TetO-Salhel-I-Sox2HMG

    Plasmid

    #231533

    Purpose

    Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    mSox2 HMG flanked with N- and C-termini of Salhel-I
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVTHM-Chim-Pchi-3xFLAG

    Plasmid

    #231536

    Purpose

    Expresses Salpingoeca helianthica chimeric Pchi in mammalian cells, for protein overexpression

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Pchi HMG flanked with N- and C-termini of mSox2
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE2-TetO-AncSoxBCEF

    Plasmid

    #231525

    Purpose

    Expresses reconstructed Ancestral SoxBCEF in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Ancestral SoxBCEF HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE2-TetO-Chim-hCic

    Plasmid

    #231529

    Purpose

    Expresses chimeric human Cic in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Human Cic HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE2-TetO-Salhel-II-Sox2HMG

    Plasmid

    #231534

    Purpose

    Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    mSox2 HMG flanked with N- and C-termini of Salhel-II
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLVTHM-Chim-Salhel-I-3xFLAG

    Plasmid

    #231535

    Purpose

    Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for protein overexpression

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Salhel-I HMG flanked with N- and C-termini of mSox2
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE2-TetO-Chim-Salhel-I

    Plasmid

    #231513

    Purpose

    Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Salhel-I HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE2-TetO-Chim-Pchi

    Plasmid

    #231514

    Purpose

    Expresses Pigoraptor chileana chimeric Sox in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Pchi HMG flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis

    Plasmid

    #228660

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from West Nile virus with an H163A - catalytically inactive mutation for xxxxx

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    H163A - catalytically inactive/West Nile Virus NS…

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pME-MCS (JDW 455)

    Plasmid

    #229832

    Purpose

    A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    attL1/L2 flanked MCS
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. N-Terminal Halo XRCC4

    Plasmid

    #207541

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. N-terminal Halo-Ku70

    Plasmid

    #207547

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with flanked by human Ku70 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 3xMS-TR-100-PURO HRD

    Plasmid

    #207582

    Purpose

    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    

    Use

    Tags

    3xMS2

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GB4405

    Plasmid

    #215256

    Purpose

    Module for the expression of nptII, the N. nambi CPH, LUZ and H3H gene flankeds by insulators on both sides

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-MAR10-SF-nptII-CPH-LUZ-H3H-MAR10-SF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204797

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. AAV-sCAG-eGFP-ACAP1

    Plasmid

    #203800

    Purpose

    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWR16

    Plasmid

    #202711

    Purpose

    integrating bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MLKS2delARMec

    Plasmid

    #130860

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Deletion of armadillo domain

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. MLKS2del4exp

    Plasmid

    #130861

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of terminal four amino acids

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. MLKS2delKASHexp

    Plasmid

    #130862

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of KASH domain

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. MLKS2delTMexp

    Plasmid

    #130863

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of transmembrane domain

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. MLKS2delARMexp

    Plasmid

    #130864

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of armadillo domain

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. MLKP2delKASH

    Plasmid

    #130838

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of KASH domain, addition of three alani…

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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44. MLKS2del4ec

    Plasmid

    #130857

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Deletion of terminal four amino acids

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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45. MLKS2delKASHec

    Plasmid

    #130858

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    eGFP-FLAG-HA

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pCAG-DIO-basic

    Plasmid

    #196882

    Purpose

    Used for preparation of double-floxed inverse ORF vectors

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Type

    Empty backbone

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBetaActin-IC2N-dCer3

    Plasmid

    #191325

    Purpose

    Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    IC2N-dCer-FRB (Dync1i2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-237

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. GB2890

    Plasmid

    #193133

    Purpose

    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1dG2b.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pQmod3S-GG

    Plasmid

    #191349

    Purpose

    Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pENT-L1-Kozak-NLSlacZ-stop-L2

    Plasmid

    #186358

    Purpose

    Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ Synthetic, E. coli)
    

    Use

    Expression of nuclear lacz reporter

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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