Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,871 results for: KIN

Clear keywords
Showing: 6001 - 6050 of 11871 results

  2. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid
    #214230
    Purpose
    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75
    Insert
    cSrc kinase SH2 domain (SRC Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214230-plasmid-map-sequence-id-429073

    Enlarge

  8. pcDNA3-Lyso-PTEN(A4)-mCherry

    Plasmid
    #184054
    Purpose
    Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.
    Depositor
    Jin Zhang
    Article
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Insert
    Lyso-PTEN(A4)-mCherry (PTEN Human)
    Tags
    Lysosome-associated membrane protein 1 (LAMP1). a…
    Expression
    Mammalian
    Mutation
    Ser 380/385 and Thr 382/383 in PTEN mutated to Al…
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184054-plasmid-map-sequence-id-363239

    Enlarge

  9. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169183
    Purpose
    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag (nsp12 C-terminus) and His6 (as internal t…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169183-plasmid-map-sequence-id-333983

    Enlarge

  13. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

    Enlarge

  15. pCAG-2dV-Camui (T305D/T306D)

    Plasmid
    #220368
    Purpose
    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    changed both Threonine 305 and 306 to aspartic ac…
    Promoter
    CAG
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220368-plasmid-map-sequence-id-469102

    Enlarge

  16. CelTOS-COMP-blac-flag-his

    Plasmid
    #111014
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    cell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111014-plasmid-map-sequence-id-224577

    Enlarge

  34. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

    Enlarge

Showing: 6001 - 6050 of 11871 results