Addgene

We narrowed to 10,121 results for: AGA

Showing: 3631 - 3660 of 10121 results

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pCK074 p5E exorh:EGFP

Plasmid

#195951

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Luciferase shRNA

Plasmid

#225343

Purpose

shRNAmir backbone for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

Luciferase shRNA (LOC116160065 Mouse)

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector

Plasmid

#99292

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.1

Plasmid

#32445

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Tags

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hPLEKHM1

Plasmid

#64146

Purpose

For protein expression in E.coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Mutation

Promoter

cspA

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

Plasmid

#83947

Purpose

gRNA cassette-carrying vector with natMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

gRNA targeting ADE2 gene

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 2

Plasmid

#36102

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 2 (GNG2 Bovine)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Article

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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768 pME mApple-CAAX

Plasmid

#195961

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGF-mSox2-ONL

Plasmid

#65719

Purpose

mSox2 reporter plasmid (expresses ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Sox2 promoter and ONL

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

Plasmid

#87694

Purpose

Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

ERT2-PhoCl-Cre-PhoCl-ERT2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 1

Plasmid

#36111

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B1 (GNB1 Bovine)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pCSIIbsr-6013x

Plasmid

#108654

Purpose

An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

Raichu-EV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-ERK-EV

Use

Tags

XFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCK083 desmaMCS:minpromEGFP,exorh:mCherry

Plasmid

#200016

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts1

Plasmid

#115886

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgPREX1

Plasmid

#86127

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

gRNA targeting PREX1 (PREX1 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-NES

Plasmid

#112714

Purpose

For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

HIV Rev nuclear export signal (NES)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

Plasmid

#220224

Purpose

AAV-STARR-seq validation control vector with Venus

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRmHA3-CH2F2-CG9866

Plasmid

#52286

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Use

Tags

2xHA 2xFLAG

Expression

Insect

Mutation

Promoter

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

Plasmid

#42046

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut

Plasmid

#42045

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Promoter

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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ER-HA-mNG21-10

Plasmid

#141165

Purpose

Split Fluorescent Reporter for ER Retrograde Trafficking

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

ER-HA-mNG21-10-KDEL

Use

Lentiviral

Tags

Signal Sequence, KDEL and HA

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

Ef1a

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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791 p3E-2A-pCS2MCSpA v2

Plasmid

#195973

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-multiple cloning site

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
Sec61b-HA-mNG21-10

Plasmid

#141213

Purpose

Membrane Tethered Split Fluorescent Reporter for ER Retragrade

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

Sec61b-HA-mNG21-10

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMK280 (mCherry2-NeoR)

Plasmid

#72796

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-erHRP(N175S mutant)

Plasmid

#79909

Purpose

erHRP(N175S mutant) pCMV backbone

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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