167,318 results

1. pEGFPC1-EPSIN 1

   Plasmid

   #22228

   Depositor

   Pietro De Camilli

   Article

   Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

   Insert

   Epsin 1 (Epn1 Rat)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   please see depositor comment below

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Expression

   Bacterial

   Promoter

   T7 promoter + lac operator

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2/11

   Plasmid

   #240486

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

   Depositor

   Meghan Rego, Addgene Research Program

   Insert

   AAV2 Rep and AAV11 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Pro-Code Vector Kit

   Plasmid Kit

   #1000000177

   Purpose

   The Pro-Code Vector Kit is a set of 120 lentiviral vector plasmids, each with a unique protein barcode (Pro-Code) and gRNA cloning site.These plasmids can be used for CRISPR screens.

   Depositor

   Brian Brown

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pJMP1356

   Plasmid

   #119276

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mNG-IRES-mScarlet-dMCP

   Plasmid

   #231089

   Purpose

   Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

   Depositor

   John Ngo

   Article

   Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

   Insert

   mNG-IRES-mScarlet-dMCP
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-APN-blast

   Plasmid

   #158455

   Purpose

   Constitutive lentiviral expression of human APN.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   APN (ANPEP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

   Plasmid

   #223164

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGL3-rev-erbα

    Plasmid

    #186823

    Purpose

    Fluorescent reporter for rev-erbα expression

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    rev-erbα promoter
    

    Use

    Luciferase

    Promoter

    rev-erbα promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

    Plasmid

    #64613

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMX229

    Plasmid

    #23002

    Depositor

    Linda Wordeman

    Article

    Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

    Insert

    Kif18A (KIF18A Human)
    

    Tags

    6His and EGFP

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2408

    Plasmid

    #143370

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXF1 (FOXF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-MITF-M

    Plasmid

    #38131

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-M (MITF Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-BEACON2

    Plasmid

    #171698

    Purpose

    Expresses BEACON2 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W98Y/W104A/Y130F

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. hE-cadherin-pcDNA3

    Plasmid

    #45769

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin (CDH1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. GFP-PH-FAPP1

    Plasmid

    #161986

    Purpose

    Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

    Insert

    PH-FAPP1 (PLEKHA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. TRE-UBC-hCD47-T2A-GFP

    Plasmid

    #205458

    Purpose

    lentiviral plasmid for expression of human CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD47 (CD47 Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-HA_N-human Prkd2

    Plasmid

    #137850

    Purpose

    Express Prkd2 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    Protein kinase d2 (PRKD2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET24_NusA-Tus

    Plasmid

    #165959

    Purpose

    Expression of soluble E. coli Tus protein

    Depositor

    Keith Pardee

    Article

    Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

    Insert

    Tus
    

    Tags

    His-NusA-Tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. 5xGAL4-TATA-luciferase

    Plasmid

    #46756

    Purpose

    Luciferase reporter gene for factors with GAL4 DNA binding domains

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Luciferase
    

    Use

    Luciferase

    Promoter

    minimal TATA

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMA4008

    Plasmid

    #70109

    Purpose

    mtDNA destruction/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    Herpes simplex virus UL12.5M185
    

    Tags

    none

    Expression

    Mammalian

    Mutation

    N-terminal deletion of UL12.5

    Promoter

    CMV and RSV

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET_RTX_(exo-)

    Plasmid

    #102786

    Purpose

    Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase with 3'-5' deficient exonuclease
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pUCmini-iCAP-AAV.MaCPNS2

    Plasmid

    #185137

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Promoter

    tight TRE

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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