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We narrowed to 26,115 results for: Nes;

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Showing: 4921 - 4960 of 26115 results

  4. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid
    #101214
    Purpose
    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C and K855A
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101214-plasmid-map-sequence-id-197223

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  5. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  7. PatoM-OG241

    Plasmid
    #72842
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoM
    Expression
    Bacterial
    Promoter
    PatoM
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72842-plasmid-map-sequence-id-133606

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  14. pCIG1_UL136_26kDa_Myc

    Plasmid
    #74955
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)
    Expression
    Mammalian
    Mutation
    delta (1-62) M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74955-plasmid-map-sequence-id-141778

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  15. pCIG1_UL136_33kda_Myc

    Plasmid
    #74957
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Myc tag (EQKLISEEDL)
    Expression
    Mammalian
    Mutation
    M63A M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74957-plasmid-map-sequence-id-141779

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  18. pGD-p19

    Plasmid
    #196326
    Purpose
    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tomato bushy stunt virus p19
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196326-plasmid-map-sequence-id-390023

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  26. pGD-γb

    Plasmid
    #196327
    Purpose
    35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    barley stripe mosaic virus γb
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196327-plasmid-map-sequence-id-390033

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  29. dCas9-ddMSK1

    Plasmid
    #165603
    Purpose
    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 S. Pyogenes, Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165603-plasmid-map-sequence-id-327022

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  33. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

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  35. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  37. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    motoneuron-specific Homeobox 9 promoter
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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Showing: 4921 - 4960 of 26115 results