169,450 results
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Plasmid#177166PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationChanged Lysine 188 to ArgininePromoterCMV with beta globin intronAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-jRCaMP1a
Plasmid#113936PurposeBacterial expression of red fluorescent Ca2+ sensor jRCaMP1aDepositorInsertjRCaMP1a
Tags6xHisExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-dDIO-MCS--4x6T
Plasmid#196422PurposeEmpty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Dre/rox; astrocyte-selectiveExpressionMammalianPromoterCAGAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
Plasmid#176283PurposeRecombinant rabies virus expressing histone-EGFPDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-FTH1
Plasmid#100749PurposePart of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tagDepositorAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC21
Plasmid#49787PurposeE. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)DepositorTypeEmpty backboneUseCloning vectorAvailable SinceJuly 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148320)
Plasmid#76378Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
px330-mcherry
Plasmid#98750PurposeCas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)
Plasmid#48296DepositorTypeEmpty backboneTagsHis6 and MBPExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH773_pTech_PlldR-HHRz-supP(opal)
Plasmid#163732PurposeLactate-inducible expression of supP(opal), preceded by a hammerhead ribozymeDepositorInserthhrz-supP
ExpressionBacterialAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
TagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialPromoterT7lacAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX301-ZIM3-KRAB-PYL1
Plasmid#154761PurposeExpresses ZIM3 KRAB domain fused to PYL1DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterCMVAvailable SinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLX-PGK-hTERT
Plasmid#114315PurposeConstitutive expression of the hTert proteinDepositorInserthTERT (TERT Human)
UseLentiviralAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab11
Plasmid#174460PurposeExpress iRFP with recycling endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with recycling endosome-targeting sequence
Tagssmall GTPase (Rab11)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh
Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3a-OSF-CypA
Plasmid#79039PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertOneSTrEP-FLAG- Cyclophilin A (PPIA Human)
TagsOneSTrEP-FLAGExpressionBacterialPromoterT7Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2515
Plasmid#91083PurposeModule C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRPromoterAtU6Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD162 (Peft-3::Cas9 + Empty sgRNA)
Plasmid#47549PurposeCas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.DepositorInsertsCas9
Empty sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available SinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mCh
Plasmid#129596PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
ExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-CCL21-V5
Plasmid#11609DepositorAvailable SinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
FUW-Luc-mCherry-puro
Plasmid#183685PurposeLentiviral vector expressing luciferase and mCherry.DepositorInsertLuc-E2A-mCherry-T2A-PuroR
UseLentiviralPromoterHuman Ubiquitin C (UbC) promoterAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsRPS27a
Plasmid#69561PurposeBacterial expression; in vitro transcription/translation (T7)DepositorAvailable SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC7A13_STOP
Plasmid#161327PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC7A13 (SLC7A13 Human)
ExpressionMammalianAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1125
Plasmid#143093PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RiboL1-jGCaMP8f
Plasmid#192612PurposeAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.DepositorInsertRiboL1-jGCaMP8f
UseAAVTags6xHisExpressionMammalianAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDriver
Plasmid#165149PurposeDriver plasmid for PROBER encoding SV40 Large T-antigenDepositorInsertSV40 Large T-antigen
ExpressionMammalianAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTA7002-avrPto
Plasmid#49156PurposePlant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPtoDepositorInsertavrPto
UsePlant transformation binary vectorPromoterdexamethasone inducibleAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148673)
Plasmid#76377Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnExpressionMammalianPromoterTet-On 3G BidirectionalAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-GIV-CTs
Plasmid#69864PurposeExpress His tagged GIV-CTs (1660–1870).DepositorInsertGIV/Girdin (CCDC88A Human)
TagsHisExpressionBacterialMutationC terminal amino acids S1660-S1870 of human GIV.PromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348APromoteraraBADAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV PHP.eB)
Viral Prep#114469-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
803 pGEX2T PTEN
Plasmid#10784DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
Src-Y530F
Plasmid#124659PurposeHuman c-Src with Y530F mutationDepositorAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF+423Dux4
Plasmid#21625DepositorInsertDouble homeobox, chr 4 (DUX4 Human)
UseLuciferaseAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCW-codon optimized catalytically inactive PHGDH
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)
Plasmid#163505PurposeDLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and humanDepositorHas ServiceAAV PHP.eBInsertSYFP2
UseAAVPromoterminBetaGlobinAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_ELAVL1
Plasmid#106105PurposeInducible gene expression of EGFP ELAVL1DepositorAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_iPE-C_P2A_Csy4
Plasmid#232428PurposeExpression plasmid for iPE-C prime editor RNPs with additional Csy4 protectionDepositorInsertiPE-C–P2A–Csy4
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGLC2-XhoI
Plasmid#99575PurposeExpression of immunoglobulin light chains in mammalian cells, human lambda 2 constant regionDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-HuC-NE2h
Plasmid#208689PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafishDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseCloning vectorPromoterHucAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6 (AAV2)
Viral Prep#83895-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsGFP (Cre-dependent)Available SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD
Plasmid#235576PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylationDepositorAvailable SinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only