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Plasmid#208965PurposeUnfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-S727A-STAT3-3xFlag
Plasmid#99262PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (S727A)DepositorInsertSignal transducer and activator of transcription 3 - S727A (Stat3 Mouse)
UseAdenoviralTags3x-FlagExpressionMutationS727APromoterCMVAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-S727D-STAT3-3xFlag
Plasmid#99263PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (S727D)DepositorInsertSignal transducer and activator of transcription 3 - S727D (Stat3 Mouse)
UseAdenoviralTags3x-FlagExpressionMutationS727DPromoterCMVAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-B103 (LM2907)
Plasmid#208968PurposeUnfused B103 DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-iCre-P2A-tdTomato
Plasmid#73351PurposeExpress iCre and tdTomato under CMV promoterDepositorInsertCMV-iCre-tdtomato
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)EGFP/CMV/WPRE∆U3
Plasmid#41790PurposeLentiviral vector for CMV promoter driven expression of EGFPDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
EW369 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236145PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
TetCMV-Pfv-Sapphire
Plasmid#203650PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSCMV:tdTomato
Plasmid#30530Purposemammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.DepositorHas ServiceCloning Grade DNAInserttdTomato
UseTagsExpressionMammalianMutationR415HPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mCherry-T2A-Puro
Plasmid#72264PurposeExpress mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.DepositorInsertmCherry-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP
Plasmid#176531PurposePiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.DepositorInsertsKGV transcription factor
TagBFP
UsePiggybac transposase integration vectorTagsErkKTR, Gal4 DNA binding domain, PEST destabiliza…ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-iCre-P2A-tdTomato
Plasmid#73351PurposeExpress iCre and tdTomato under CMV promoterDepositorInsertCMV-iCre-tdtomato
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)
Plasmid#139990PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationVQR=D1135V/R1335Q/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)
Plasmid#139992PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationVRQR=D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)
Plasmid#139991PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationVRER=D1135V/G1218R/R1335E/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)
Plasmid#139993PurposeCMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationxCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)
Plasmid#139995PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationHF1=N497A/R661A/Q695A/Q926APromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29(+exo) (LM2759)
Plasmid#208964PurposeUnfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-FFluc-GPD1L-3'UTR Full mut
Plasmid#32489DepositorInsertGPD1L 3'UTR (Gpd1l Mouse)
UseTagsLuciferaseExpressionMammalianMutationMutated in the entire miR-210 binding regionPromoterCMVAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-FFluc-GPD1L-3'UTR Seed Mut
Plasmid#32490DepositorInsertGPD1L 3'UTR (Gpd1l Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV
Plasmid#16403PurposeShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV
Plasmid#16405PurposeShuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-hRad51
Plasmid#125570PurposeExpresses the Rad51 protein in mammalian cells under CMV promoterDepositorInsertRad51 (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV plasmid
Plasmid#169739PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Puro
Plasmid#135689PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Puro
Plasmid#135687PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shRen-Blast
Plasmid#135684PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Blast
Plasmid#135686PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shTHUMPD3-Blast
Plasmid#135690PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selectionDepositorInsertshRosa26
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only