We narrowed to 963 results for: Streptococcus

1. pFW11-ffluc

   Plasmid

   #128487

   Purpose

   Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

   Depositor

   Thomas Proft

   Article

   Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

   Insert

   firefly luciferase gene
   

   Use

   Allelic replacement vector

   Tags

   spy0535 downstream flanking region and spy0535 up…

   Expression

   Mutation

   Promoter

   synthetic

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX165-eSpCas9

   Plasmid

   #140564

   Purpose

   Expresses eSpCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   K848A K1003A R1060A

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ824

   Plasmid

   #39314

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPaCas9

   Plasmid

   #111819

   Purpose

   Expresses Cas9 in T. brucei (2T1) cells

   Depositor

   David Horn

   Article

   Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-J6-dCas9-24XGP41-J9

   Plasmid

   #202006

   Purpose

   MoClo level 0 dCas9-24XGP41 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-24XGP41
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GS linkers

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-HypaCas9

   Plasmid

   #140567

   Purpose

   Expresses HypaCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   N692A M694A Q695A H698A

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Yeast_Target-AID3S

   Plasmid

   #188646

   Purpose

   Expresses Target-AID3S in yeasts

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Yeast

   Mutation

   D10A for SpCas9

   Promoter

   pGal1

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC2_7_dCas9_HC_sgRNA

   Plasmid

   #163711

   Purpose

   High copy plasmid with dCas9 and sgRNA expression cassettes

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX165-evoCas9

    Plasmid

    #140569

    Purpose

    Expresses evoCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    M495V Y515N K526E R661Q

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPOR0SP0013

    Plasmid

    #117521

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

    Plasmid

    #50031

    Purpose

    Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    PM-SrtA(strep)
    

    Use

    Tags

    C-HA

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PV247

    Plasmid

    #132338

    Purpose

    CasA constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    CasA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK17-02

    Plasmid

    #99604

    Purpose

    Plasmid containing parBp(mut)-m(sf)gfp fusion

    Depositor

    Morten Kjos, Jan-Willem Veening

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    parBp(mut)
    

    Use

    Synthetic Biology

    Tags

    m(sf)gfp

    Expression

    Mutation

    internal parS-site mutated

    Promoter

    PczcD2

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL1P3_AtU626Ter26

    Plasmid

    #191771

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL11197

    Plasmid

    #191784

    Purpose

    SpCas9 expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas9 +13 introns-T-E9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTricHis-ILY-prepore

    Plasmid

    #160519

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTricHis-ILY-WT-Y336W

    Plasmid

    #160515

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-WT-Y336W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y336W

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

    Plasmid

    #50032

    Purpose

    Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    HA-SrtA(strep)
    

    Use

    Tags

    HA-HDEL

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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