167,408 results

1. pWZL Blast GFP

   Plasmid

   #12269

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   EGFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-24d-Flag-SKP1

   Plasmid

   #213690

   Purpose

   Expresses human SKP1 in bacteria cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1 (SKP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Flag beta-2-adrenergic-receptor

   Plasmid

   #14697

   Depositor

   Robert Lefkowitz

   Article

   Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   B2AR (ADRB2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGs-NLS-TIR1_P2A_NES-TIR1

   Plasmid

   #117699

   Purpose

   Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Inserts

   TRANSPORT INHIBITOR RESPONSE 1
   TRANSPORT INHIBITOR RESPONSE 1
   

   Tags

   HA tag, Myc-NES, and NLS

   Expression

   Mammalian

   Mutation

   codon optimized for murine expression

   Promoter

   None, 2A peptide from porcine teschovirus-1 polyp…

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-BirA-ER

   Plasmid

   #20856

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2008 May . 5(5):397-9.

   Insert

   BirA-ER
   

   Expression

   Mammalian

   Mutation

   C-terminal ER targeting sequence (KDEL)

   Available Since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUSEss-CHIg-mG3_M18

   Plasmid

   #82356

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
   

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCM-CLYBL-hNIL

   Plasmid

   #105841

   Purpose

   Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-Luc-ZsGreen

   Plasmid

   #39196

   Depositor

   Bryan Welm

   Inserts

   Firefly Luciferase Luc2P
   ZsGreen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha and EF1-alpha

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGPN-TMPRSS2

    Plasmid

    #187484

    Purpose

    Expression vector - TMPRSS2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-ERBB2

    Plasmid

    #116734

    Purpose

    Lentiviral expression of ERBB2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-c2X_MBP-CLmN-H6

    Plasmid

    #234487

    Purpose

    Maltose binding protein N-terminally fused to His-tagged CLmN split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    N-terminal Fragment of the CLm Split Intein
    

    Tags

    Hexahistidine Tag and Maltose Binding Protein (MB…

    Expression

    Bacterial

    Mutation

    T69K, F75H, M118N within the N-terminal fragment …

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18T-mini-Tn7T-Tp-dsRedExpress

    Plasmid

    #65036

    Purpose

    TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. eMagAF-EGFP-PM

    Plasmid

    #162247

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

17. PB-GFP-Gal8

    Plasmid

    #127191

    Purpose

    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Insert

    GFP-Gal8 (LGALS8 Human)
    

    Tags

    Gal8 is fused to the c-terminus of GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRE4-Azi

    Plasmid

    #105829

    Purpose

    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

    Depositor

    Irene Coin

    Article

    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

    Inserts

    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    

    Expression

    Mammalian

    Mutation

    (Y37L, D182S, F183M, L186A, D265R)TyrRS

    Promoter

    PGK and U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. nLuc-GLP-1R-His-FLAG

    Plasmid

    #124831

    Purpose

    Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

    Depositor

    Sam Gellman

    Article

    Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

    Insert

    GLP1R (GLP1R Human, Synthetic)
    

    Use

    Luciferase

    Tags

    FLAG, His6, and NanoLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR8k-GFPuv

    Plasmid

    #106385

    Purpose

    L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA5FRT/TO_HA-mAID-nanobody

    Plasmid

    #117713

    Purpose

    Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBBR1k-J23107-GFPuv

    Plasmid

    #106389

    Purpose

    pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral Prep

    #104492-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

24. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-hsyn-ASAP5-Kv2.1-WPRE

    Plasmid

    #225707

    Purpose

    Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Has Service

    AAV1

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Promoter

    human synapsin

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLG010 (DRT type 2)

    Plasmid

    #157888

    Purpose

    Expresses the DRT type 2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 2
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pK18msB

    Plasmid

    #177839

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

    Depositor

    Christopher Johnson

    Article

    Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDONR223-MAP2K1

    Plasmid

    #23406

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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29. myc-hULK1

    Plasmid

    #31961

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (ULK1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV6-Neo-ISG15-LRAA

    Plasmid

    #80405

    Purpose

    overexpression of nonconjugative ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Expression

    Mammalian

    Mutation

    nonconjugative ISG15, LRAA

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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