We narrowed to 9,373 results for: Pol;

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pCMV5-Flag-RhoA wt

Plasmid

#11750

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE

Plasmid

#218760

Purpose

Provides AND intersectional (Cre+Flp) expression of ArchT-eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

ArchT

Use

AAV

Tags

eGFP

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Article

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.3.0 Empty

Plasmid

#82447

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pB80-2xFKBP-L-mCherry-CAAX

Plasmid

#193713

Purpose

Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

2xFKBP-L-mCherry-CAAX

Tags

2xFKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCsA

Plasmid

#136067

Purpose

Acceptor plasmid for even level position 1, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pmCherry-C1-p63RhoGEF

Plasmid

#67896

Purpose

Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-p63RhoGEF

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 beta-catenin WT

Plasmid

#16071

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

*

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag Runx1 FL (P#1790)

Plasmid

#14585

Depositor

Matija Peterlin

Article

Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

Insert

Runx1 (Runx1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pBa.Lamp1-GFP

Plasmid

#180250

Purpose

Mammalian expression of LAMP1

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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NylCK-TS_pET28a

Plasmid

#215423

Purpose

Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK-TS

Expression

Bacterial

Mutation

S111G/A137L

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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RP185 pUAST EGFP:Or47b

Plasmid

#59525

Purpose

N-terminal fusion of GFP to Drosophila melanogaster Or47b

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Or47b (Or47b Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
Ki67∆FHA-mNeonGreen-IRESpuro2

Plasmid

#183739

Purpose

Expresses Ki67∆FHA-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67_lacking FHA (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67(lacking FHA)

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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MD-2

Plasmid

#20864

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Myeloid differentiation factor 2 (LY96 Human)

Tags

protein A tag

Expression

Insect

Mutation

contains amino acids 19–160

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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YFC43

Plasmid

#89488

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP

Depositor

Alejandro Ferrando

Article

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-C (aa 154-240)

Expression

Plant

Promoter

2x35S

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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YFN43

Plasmid

#89487

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

Depositor

Alejandro Ferrando

Article

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-N (aa 1-154)

Expression

Plant

Promoter

2x35S

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAUC40

Plasmid

#71298

Purpose

Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

Depositor

Susanne von Bodman

Article

Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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HSP70-pp7i-mCh-UPG

Plasmid

#161011

Purpose

C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET30a-GlmU-NahK-ET64

Plasmid

#234663

Purpose

Expression an ELP-tagged fusion enzyme of GlmU and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

N-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_SpCas9_USP7 Exon 3 gRNA

Plasmid

#131257

Purpose

pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

humanised S. pyogenes Cas9 nuclease

Expression

Mammalian

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-lacZ

Plasmid

#72948

Purpose

Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 0 - Bioparts

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCk3

Plasmid

#136697

Purpose

Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight

Plasmid

#205077

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

Plasmid

#126553

Purpose

Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

Thy1.2 and GFP

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge