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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ13960 tyrosinase(Priestia megaterium)

Plasmid

#239454

Purpose

express tyrosinase protein and melanin

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

tyrosinase

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry_SeV_Nucleoprotein

Plasmid

#228806

Purpose

Expression of Sendai virus Nucleoprotein

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

Nucleoprotein

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_SeV_RdRP

Plasmid

#228808

Purpose

Expression of Sendai virus RdRP

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

RdRP

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-RhoA

Plasmid

#12202

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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piRFP_SeV_Phosphoprotein

Plasmid

#228807

Purpose

Expression of Sendai virus Phosphoprotein

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

Phosphoprotein

Tags

iRFP670

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry

Plasmid

#207668

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP)

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

MCP-mCherry

Expression

Mammalian

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM22082_sfGFP1-10

Plasmid

#153515

Purpose

E.coli expression plasmid for the production of GFP1-10

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

sfGFP1-10

Expression

Bacterial

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-FKP-ET64

Plasmid

#234665

Purpose

Expression an ELP-tagged FKP enzyme

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Insert

fucokinase/GDP-fucose pyrophosphorylase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCYP64

Plasmid

#212897

Purpose

Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Mutation

P450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Grx1-RoTq-On

Plasmid

#234467

Purpose

Mammalian expression of Grx1-RoTq-On

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

Grx1-RoTq-On

Use

AAV

Expression

Mammalian

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP TFEB-sfGFP

Plasmid

#135402

Purpose

Expresses TFEB-sfGFP

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

TFEB (TFEB Human)

Use

Retroviral

Tags

sfGFP

Expression

Mammalian

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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negMaLionR

Plasmid

#113909

Purpose

negative control for Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#82400

Purpose

Gateway-compatible zebrafish elavl3 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

elavl3 promoter

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2-mut

Plasmid

#192783

Purpose

control probe of G-Flamp2, insensitive to cAMP

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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2C-EGFP

Plasmid

#69071

Purpose

Expresses EGFP under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

EGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB402-er-mCherry

Plasmid

#186288

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-PSP-hTG

Plasmid

#85470

Purpose

bacterial expression of human tissue transglutaminase

Depositor

Jeffrey Keillor, Ray Truant

Article

Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

Insert

hTG2 (TGM2 Human)

Tags

GST and PSP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Green Glifon600

Plasmid

#126207

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon600

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1-GFP (27)

Plasmid

#26061

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6

Plasmid

#41596

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-Cdc42

Plasmid

#12201

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCF1135

Plasmid

#222106

Purpose

E. coli - Veillonella shuttle plasmid with single selectable marker

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SGL40C.EFS.dTomato

Plasmid

#89395

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pWS3799-gRNA-Csy4-template

Plasmid

#182827

Purpose

SpCas9 gRNA-Csy4 template

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

SpCas9 gRNA scaffold + Csy4 sequence

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 Stu2 1-590

Plasmid

#38314

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 1770 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pHA-eIF4E

Plasmid

#17343

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

eIF4E (EIF4E Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor14-ShHELIX-KanR (CJT70)

Plasmid

#181793

Purpose

pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYMA3_SidK_GFP

Plasmid

#184276

Purpose

Legionella pneumophila SidK (1-278) - GFP

Depositor

John Rubinstein

Article

Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

Insert

SidK (1-278) (sidK Legionella pneumophila)

Tags

GFP

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ELP-TEMP/pcDNA3.1(-)

Plasmid

#178434

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

Insert

ELP-TEMP

Tags

No tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-3HA-TRP1

Plasmid

#41599

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA and yeast ADH1 terminator

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV0021 [15ARE23bp-GFP]

Plasmid

#246420

Purpose

AHR GFP reporter plasmid in a lentiviral backbone

Depositor

Marco Jost

Article

Verma et al bioRxiv [Preprint]. 2025 Sep 15:2025.09.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

AHR responsive minimal promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-Lantern-HaloTag-NES

Plasmid

#250919

Purpose

Expresses Lantern in mammalian cytosol

Depositor

Peng Zou

Article

Fang et al Research Square 2025 [Preprint]. https:/

Insert

Lantern

Use

Lentiviral

Tags

HaloTag-NES and V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-HHARI

Plasmid

#17450

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 5, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
543-p3E-2A-mcherrypA

Plasmid

#26031

Depositor

Nathan Lawson

Article

Villefranc et al Development. 2013 Apr;140(7):1497-506. d

Insert

mCherry

Use

Entry vector

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDV02

Plasmid

#212156

Purpose

Plasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Article

Tan et al ACS Synth Biol. 2023 Jul 21;12(7):2041-2

Insert

secondary alcohol dehydrogenase

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pPLV_C1

Plasmid

#186415

Purpose

Endogenous murine RNase inhibitor production for cell-free lysate

Depositor

Jerome Bonnet

Article

Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

Insert

Murine RNase Inhibitor

Use

Synthetic Biology

Promoter

T7

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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