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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2

Plasmid

#42175

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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miniSOG-Mito-7

Plasmid

#57773

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pETDuet S2

Plasmid

#171921

Purpose

Express RNA-based (p)ppGpp sensor and a NirFP

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Inserts

RNA-based (p)ppGpp sensor
NirFP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)

Viral Prep

#137148-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

nEF

Tags

EYFP (Cre and Flp-dependent)

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only
Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Lifeact-EGFP

Plasmid

#67303

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mGreenLantern

Plasmid

#164469

Purpose

Constitutive CAG-driven expression of mGreenLantern in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

AAV

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CAG

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pmGFP10C-Tau

Plasmid

#71433

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Tags

GFP (1-212)

Expression

Mammalian

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

Plasmid

#60606

Purpose

Mammalian Display vector

Depositor

Wayne Marasco

Article

Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

Insert

PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-Sec8

Plasmid

#53758

Purpose

pEGFP-C3 plasmid that expresses Sec8

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec8 (EXOC4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 CDK7 D155A HA (P#812)

Plasmid

#14648

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Mutation

D155A: dominant negative

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pBG100-TCPTP

Plasmid

#33365

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Tags

6xHIS - cleavable

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pUC-H1-gRNA

Plasmid

#61089

Purpose

CRISPR/Cas9 system gRNA cloning

Depositor

Shenglin Huang

Article

Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

Insert

H1 promoter; gRNA sequences

Use

CRISPR; /cas9 grna cloning vector

Expression

Mammalian

Promoter

H1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pVSVG-PATagRFP

Plasmid

#31947

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

VSVG

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pMx-puro-MGT

Plasmid

#111809

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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E12-pCLBabe

Plasmid

#20918

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 (TCF3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G155D

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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Pmec-18 caspase-3 (p12)::nz [TU#813]

Plasmid

#16082

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

caspase-3 (p12) :: nz (CASP3 Human)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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