We narrowed to 13,608 results for: crispr cas9

1. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

   Plasmid

   #228252

   Purpose

   pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   SpCas9 P23H sgRNA, mTagBFP2
   

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223321

   Purpose

   It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   SpCas9 codon optimized for human (cas9 Synthetic, Human)
   chloramphenicol resistance protein
   CcdB
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

   Plasmid

   #112214

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Mutation

   D10A,H840A,N863A

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

   Plasmid

   #112215

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Mutation

   D10A,H840A,N863A,C710S

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

   Plasmid

   #197505

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

   Promoter

   CMV and T7

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fuw-dCas9-Tet1CD-P2A-BFP

   Plasmid

   #108245

   Purpose

   Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A

   Promoter

   Ubc

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

   Plasmid

   #209298

   Purpose

   A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.

   Depositor

   Birgitt Schuele

   Article

   Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1

   Inserts

   dCas9
   tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetracycline-inducible promoter (TRE3G)

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP457 pGK-dCas9-Suntag-BFP

    Plasmid

    #100957

    Purpose

    BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA and BFP

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Po (phoenol-inducible promoter)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9(1.1)-ABL1-gRNA1-exon4

    Plasmid

    #163322

    Purpose

    eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 1_Exon4
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

14. eSpCas9(1.1)-ABL1-gRNA2-exon4

    Plasmid

    #163323

    Purpose

    eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 2_Exon4
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

15. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

    Plasmid

    #223163

    Purpose

    2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

    Plasmid

    #192132

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-Y
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (Y446A)

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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