We narrowed to 3,489 results for: psin

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Synthetic, Human)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Promoter

U6 promoter;

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

Plasmid

#178827

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, HA

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-nEF-Con/Fon-bREACHes-EYFP

Plasmid

#137145

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Con/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

Plasmid

#105982

Purpose

This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

Depositor

Marcel Bruchez

Article

Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

Insert

FLEX-FAPdL5-POST-T2A-dTomato.FLEX

Use

AAV and Cre/Lox

Tags

FAPdL5-POST, T2A-dTomato, and myc

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA

Plasmid

#210503

Purpose

expresses LAUNCHERsbmv component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

Plasmid

#22032

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

Plasmid

#145263

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+9A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-ChR2-YFP

Plasmid

#178725

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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ECON

Plasmid

#82553

Purpose

Epigenetic effector construct for targeted methylation

Depositor

Joseph Irudayaraj

Article

Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)

Tags

HALO and mCherry

Expression

Mammalian

Mutation

N-terminal photolyase homology region domain of C…

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hNCL

Plasmid

#103805

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hNCL (CIB1, NCL Mustard Weed, Human)

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hNCL

Plasmid

#103806

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hNCL (CIB1, NCL Mustard Weed, Human)

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-dsm

Plasmid

#89743

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi dark-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-lsm

Plasmid

#89742

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi lit-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

Plasmid

#118622

Purpose

expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7-E6 3'UTR (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed ttgcttt to cggtaag (496 - 502 nt in 3…

Promoter

synapsin I (rat)

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Promoter Only Target

Plasmid

#127669

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

EF1a and hU6

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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