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pAN-PTet-dCas9

Plasmid

#62244

Purpose

controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

catalytically inactive, D10A, H840A

Promoter

Ptet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLN-AIDA-Tyr1

Plasmid

#192831

Purpose

IPTG inducible AIDA-Tyrosinase expression

Depositor

André Studart

Article

Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

Insert

AIDA-Tyr1

Expression

Bacterial

Promoter

pL-lac

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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T2A 3xNLS GFP

Plasmid

#227721

Purpose

Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-G418

Plasmid

#160215

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HPH

Plasmid

#160216

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-VASP-5

Plasmid

#57433

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

VASP

Tags

mEos2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CSII-prEF1a-mCherry-3xNLS

Plasmid

#125262

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

mCherry-3xNLS

Use

Lentiviral

Tags

3xNLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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hsgk1

Plasmid

#83432

Purpose

full length human sgk1 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

Insert

human Sgk1 (SGK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27

Plasmid

#163617

Purpose

mouse SNX27 cDNA N-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBZS-M.CviQIX (NDel15)

Plasmid

#198357

Purpose

Express M.CviQIX (NDel15) in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviQIX

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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Plas-gRNA-Z3EV

Plasmid

#157659

Purpose

GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

GFP-gRNA NatMX Z3EV

Use

Insert storage (replicative in e. coli)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBZS-M.CviPII (NDel15)

Plasmid

#198356

Purpose

Express M.CviPII (NDel15) in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviPII

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKI1.1R

Plasmid

#85808

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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5xGAL4-TATA-luciferase

Plasmid

#46756

Purpose

Luciferase reporter gene for factors with GAL4 DNA binding domains

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Luciferase

Use

Luciferase

Promoter

minimal TATA

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Article

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only