167,514 results

1. pDONR223_MYC_WT

   Plasmid

   #82927

   Purpose

   Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, David Root, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   Myc (MYC Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LAMP1-mGFP

   Plasmid

   #34831

   Depositor

   Esteban Dell'Angelica

   Article

   Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

   Insert

   LAMP1 (LAMP1 Human)
   

   Tags

   GSTGSTGSTGA linker, Kozak, and mGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLBW cox8 EGFP mCherry

   Plasmid

   #78520

   Purpose

   Fluorescent mitophagy reporter

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   none
   

   Use

   Retroviral

   Tags

   cox8 EGFP mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_tre_Cas9

   Plasmid

   #126029

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible Cas9

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   humanized S. pyrogenes Cas9
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Promoter

   EF1 alpha and TRE

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRH-1363 LV EF1a-CD19 IRES-EGFP

   Plasmid

   #201919

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0144

   Plasmid

   #141536

   Purpose

   Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PHOX2A (PHOX2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBI-p53 R175H/EGFP

   Plasmid

   #16544

   Depositor

   Bert Vogelstein

   Article

   Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

   Insert

   p53 (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R175H

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CMV.ffLuciferase.SV40 (AAV8)

    Viral Prep

    #105532-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV.CMV.ffLuciferase.SV40 (#105532). In addition to the viral particles, you will also receive purified pAAV.CMV.ffLuciferase.SV40 plasmid DNA. CMV-driven ffLuciferase control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CMV

    Tags

    ffLuciferase

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiGuide-puro-NLS-GFP

    Plasmid

    #185473

    Purpose

    Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pmScarlet-I3_C1

    Plasmid

    #189756

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep

    #162376-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

15. TurboID-mNeongreen-3xFLAG fusion pos3

    Plasmid

    #172864

    Purpose

    C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

    Depositor

    Mario de Bono

    Article

    Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

    Insert

    TurboID
    

    Use

    Unspecified

    Tags

    FLAG, mNeongreen, and myc

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX_TRC311

    Plasmid

    #113668

    Purpose

    Gateway destination vector for lentiviral expression

    Depositor

    John Doench

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PBZ-mRuby2

    Plasmid

    #110650

    Purpose

    expresses a PAR-binding (PBZ) domain fused to mRuby2

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. nuclear NAD+ biosensor

    Plasmid

    #186789

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW2066

    Plasmid

    #236575

    Purpose

    ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Expression

    Plant

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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