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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-C

Plasmid

#183160

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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5HT2A-APEX2

Plasmid

#215558

Purpose

Stable, inducible expression of 5HT2A

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago1

Plasmid

#21533

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pB-dCas9-PBE

Plasmid

#98159

Purpose

Expresses CRISPR base editor in maize

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Maize

Expression

Plant

Promoter

Ubi

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-cytoFLARE1.0/1.1-TF

Plasmid

#234516

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-FLAG-tTA

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19/eCFP-C

Plasmid

#183156

Purpose

Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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RCAS-myrAkt

Plasmid

#11547

Depositor

Sandra Orsulic

Article

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

Protein Kinase B (Akt1 Mouse)

Use

Retroviral

Tags

HA tag and myr signal

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
5'-3xFLAG minimal CMV pCDNA5

Plasmid

#212044

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-VAMP2:HRP

Plasmid

#60659

Purpose

Labels synaptic vesicles for electron microscopy

Depositor

Scott Sternson

Article

Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

Insert

VAMP2:HRP

Use

AAV and Cre/Lox

Tags

Myc

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pIVT/Smarca4

Plasmid

#122146

Purpose

For making Smarca4 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarca4 (Smarca4 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET-NEMO

Plasmid

#11966

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

His

Expression

Bacterial

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pXJ-His-PDCD1LG2

Plasmid

#232660

Purpose

transient expresses PD-L2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

PDCD1LG2 (PDCD1LG2 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMT2-HA-MKK3

Plasmid

#21580

Depositor

John Kyriakis

Article

Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

Insert

mitogen-activated protein kinase kinase 3 (MAP2K3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pNK6032

Plasmid

#219738

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter FMV

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

Figwort mosaic virus (FMV) promoter

Use

Synthetic Biology

Expression

Plant

Promoter

FMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag YAP127/128/131/381A

Plasmid

#27378

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

S127/128/131/381A

Available Since

March 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAAP003l2

Plasmid

#209748

Purpose

RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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NHA-GW182

Plasmid

#36438

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 254-1962 only

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pED-hIRE1(I642G)

Plasmid

#16263

Depositor

Peter Walter

Article

Lin et al Science. 2007 Nov 9. 318(5852):944-9.

Insert

IRE1 (ERN1 Human)

Expression

Mammalian

Mutation

isoleucine to glycine mutation at residue 642

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)

Plasmid

#187067

Purpose

Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmaxNG-Gp41

Use

AAV and CRISPR

Expression

Mammalian

Promoter

MHP1

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pYL322d1/eGFP-C

Plasmid

#183152

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-mIFNγ

Plasmid

#230004

Purpose

Gal4-dependent expression of secreted mouse IFNγ

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-mIFNγ (Ifng Synthetic)

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-Craf R401H

Plasmid

#118334

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf R401H (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R401H

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)

Plasmid

#229775

Purpose

enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Matveev et al bioRxiv [Preprint]. 2024 Nov 1:2024.11.0

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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A3A-Gam

Plasmid

#119769

Purpose

Conversion of C to T monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Craf SSDD

Plasmid

#118337

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf SSDD (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

SSDD

Promoter

CMV

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSW457 - tCLV3

Plasmid

#115980

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSW455 - pCLV3

Plasmid

#115979

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSM_ser-2prom3p::myr-mCherry

Plasmid

#164223

Purpose

Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

Depositor

Yan Zou

Article

Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

Insert

myr-mCherry

Expression

Worm

Promoter

ser-2promo3

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pVL26 - pSCR

Plasmid

#116021

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pSCR

Use

Unspecified

Mutation

T171A by SDM to abolish Eco31I site

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 KRAB

Plasmid

#188500

Purpose

Expresses FLAG tagged HiFi dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pF208wt-luc

Plasmid

#122143

Purpose

For luciferase expression

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Fam208a promoter (Tasor Mouse)

Use

Luciferase

Promoter

Fam208a

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 delta linker

Plasmid

#32825

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D and amino acids 752-1076 deleted

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-ZnF/HIT1

Plasmid

#15332

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

zinc finger, HIT domain containing 1 (ZNHIT1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
MLS-mir-17-92

Plasmid

#64090

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-AMOT p130 deltaEYLI

Plasmid

#32826

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

E5D, deletion of last 4 amino acids (EYLI)

Promoter

CMV

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pNK869

Plasmid

#219737

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7

Use

Synthetic Biology

Expression

Plant

Promoter

AtPD7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW614 - pREV

Plasmid

#115989

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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