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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP (PV2527)

Plasmid

#99039

Purpose

AAV-mediated expression of ArchT under the CamKII promoter

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

TET-On

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 K821R

Plasmid

#59813

Purpose

Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

K821R

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAL130

Plasmid

#22475

Purpose

Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF3NT (PIF3 Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Expression

Plant

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJS145

Plasmid

#179159

Purpose

Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

Depositor

Iva Greenwald

Article

Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Insert

rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR

Expression

Worm

Mutation

Flexon replacing 2nd intron of 2xNLS::GFP

Promoter

rps-27p

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-E12

Plasmid

#25990

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 WT

Plasmid

#59812

Purpose

Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep164

Plasmid

#136873

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep164 (CEP164 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-MyoD

Plasmid

#25992

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-MyoD homodimer (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only
pSMP-Ehmt1_2

Plasmid

#36336

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

Plasmid

#154229

Purpose

Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)

Depositor

Arnold Driessen

Article

Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

Insert

p40S:QfDBD-VP16AD-GFP-NLS

Use

Synthetic Biology; Fungal expression

Tags

GFP-NLS; DsRed-SKL

Expression

Bacterial

Promoter

p40S (AN0465 A. nidulans)

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment A

Use

Luciferase

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#171117

Purpose

Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#163698

Purpose

AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

Depositor

Gina Turrigiano

Article

Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

Insert

CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mammalian

Promoter

CaMKII fragment

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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eDrcKIT

Plasmid

#184001

Purpose

Optically controlled eDrcKIT expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrcKIT (KIT Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mNeonGreen

Plasmid

#196869

Purpose

Expression of the gamma-tubulin fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mNeonGreen (Tubg1 Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet1 promoter B

Plasmid

#63882

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment B

Use

Luciferase

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_V12

Plasmid

#39516

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
PHR-GFP-p65

Plasmid

#183929

Purpose

Optogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-p65 (CRY2 Synthetic, Mustard Weed)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12/pSIR

Plasmid

#61080

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

gRNA_hIRF1 promoter #12 (IRF1 Human)

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-A3A-seBE4max-IRES

Plasmid

#174698

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

A3A-seBE4max-IRES (APOBEC3A Human)

Tags

myc tag

Expression

Mammalian

Mutation

A3A-M13I, UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2MT-mbeta-catenin delta6

Plasmid

#100883

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 489-781aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET a sfMatryoshCaMP6s-T78H

Plasmid

#100021

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfMatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP and T78H m…

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2MT-mbeta-catenin delta7

Plasmid

#100884

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 668-781aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2MT-mbeta-catenin delta5

Plasmid

#100882

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 400-666aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMP-Ehmt1_4

Plasmid

#36338

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCS2MT-mbeta-catenin delta2

Plasmid

#100879

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 1-276aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2MT-mbeta-catenin delta1

Plasmid

#100878

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 1-150aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1Xpress-Bcr-HisB

Plasmid

#32508

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Tags

HISB

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Ehmt1_1

Plasmid

#36335

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pEBTetDBl-CLIP-CP110

Plasmid

#136852

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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