We narrowed to 17,535 results for: puromycin

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MSCV CreERT2 puro

Plasmid

#22776

Purpose

Retroviral expression of tamoxifen-inducible Cre-ERT2

Depositor

Tyler Jacks

Article

Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro

Plasmid

#85132

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLIX_403

Plasmid

#41395

Purpose

Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

V5

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-Sox2-Pur

Plasmid

#16577

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA H1047R

Plasmid

#12524

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H1047R. Tumor-derived allele.

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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Lox-Stop-Lox TOPO

Plasmid

#11584

Purpose

Cre-dependent activation of gene of interest by removing Stop sequences; Puro selection.

Depositor

Tyler Jacks

Article

Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

Insert

loxP-STOP-loxP

Use

Cre/Lox

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pBABEpuro HA Brca1

Plasmid

#14999

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

Brca1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro DEST (w529-2)

Plasmid

#19068

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-Nanog-Pur

Plasmid

#16578

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R (tumor-derived)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-Oct4-Pur

Plasmid

#16579

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-LIN28-Pur

Plasmid

#16580

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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Ef1a_Large T-antigen_Ires_Puro

Plasmid

#18922

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

Large T Antigen (SV40gp6 Simian virus 40)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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LentiGuide Cyclone-Puro P2A EGFP

Plasmid

#247497

Purpose

Acyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion protein

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

puromycin n-acetyltransferase with Cyclone insertion

Use

Lentiviral and Synthetic Biology

Tags

Puro(Cyclone)-P2A-EGFP

Expression

Mammalian

Mutation

Cyclone was inserted after Glutamine135 of puromy…

Promoter

EF-1a

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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