Addgene

We narrowed to 16,434 results for: puromycin

Showing: 281 - 300 of 16434 results

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

Plasmid

#162117

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to ProtC-FLAG-AU1

Use

Lentiviral

Tags

ProtC-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-AU1

Plasmid

#162107

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-HA-AU1

Use

Lentiviral

Tags

VSVg-HA-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

Plasmid

#162108

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-FLAG-AU1

Use

Lentiviral

Tags

VSVg-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-FLAG

Plasmid

#162112

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-HA-FLAG

Use

Lentiviral

Tags

StrepTagII-HA-FLAG

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

Plasmid

#162084

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG

Use

Lentiviral

Tags

VSVg-ProtC-FLAG

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA

Plasmid

#162089

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-ProtC-HA

Use

Lentiviral

Tags

StrepTagII-ProtC-HA

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

Plasmid

#162091

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1

Use

Lentiviral

Tags

StrepTagII-ProtC-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA

Plasmid

#162080

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-StrepTagII-HA

Use

Lentiviral

Tags

VSVg-StrepTagII-HA

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

Plasmid

#154881

Purpose

Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2
BCL2 (BCL2 Human)
miR9/9*-124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

Plasmid

#162098

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to HA-FLAG-AU1

Use

Lentiviral

Tags

HA-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

Plasmid

#162099

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC

Use

Lentiviral

Tags

VSVg-StrepTagII-ProtC

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

Plasmid

#68345

Purpose

A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

Depositor

Jannik Elverløv-Jakobsen

Article

Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

Inserts

puromycin
hCas9

Use

CRISPR; Flp/frt

Tags

linked to red flouresent protein gene mKateII and…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP

Plasmid

#60511

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur

Plasmid

#60523

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSIH1-puro-control shRNA

Plasmid

#26597

Purpose

Lentiviral control shRNA vector with puromycin selection.

Depositor

Frank Sinicrope

Article

Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP

Plasmid

#60513

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge