169,450 results
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Plasmid#182352PurposePan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-GFP-IMP2-2
Plasmid#42175DepositorInsertInsulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
TagsGFPExpressionMammalianPromoterT7Available SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJOE6261.2
Plasmid#135079PurposeP. putida KT2440 rescue constructDepositorInsertupp (upp Pseudomonas putida)
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVβ-p300-myc
Plasmid#30489PurposeMammalian expression of human p300 with myc tagDepositorAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179120PurposeExpresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertcontrol sgRNA
UseAAVPromoterU6Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-C1
Plasmid#54650PurposeLocalization: C1 Cloning Vector, Excitation: 549, Emission: 565DepositorTypeEmpty backboneTagsmOrange2ExpressionMammalianAvailable SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_VHL_WT
Plasmid#81874PurposeGateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV5 trial size)
Viral Prep#37825-AAV5.TPurposeReady-to-use AAV5 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-GFP.W
Plasmid#51010PurposeLentivector that expresses LifeAct-GFP from internal human PGK promoterDepositorInsertLifeAct-GFP
UseLentiviralTagsLifeActExpressionMammalianPromoterhuman PGKAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV1.X1
Plasmid#209780Purposenon-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV1-X1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV9-X1.1)
Viral Prep#37825-AAV9-X1.1PurposeReady-to-use AAV9-X1.1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the AAV9-X1.1 serotype, which permits efficient transduction of brain endothelial cells. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo largeTcDNA
Plasmid#1780PurposeRetroviral expression of Large T Antigen. Used to create immortalized cell lines.DepositorAvailable SinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
L444P GBA pcDNA3.1
Plasmid#188582PurposeEctopic expression L444P GBADepositorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-NRASG12V/D38A-IRES-mVenus
Plasmid#236077PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).DepositorInsertsUseSleeping beauty transposon plasmidMutationG12V D38APromoterCAGGSAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
cyto-Ruby3-iATPSnFR1.0
Plasmid#102551Purposeexpresses cytoplasmic ATP sensor in mammalian cells with red reference proteinDepositorInsertRuby3-iATPSnFR1.0
TagsRuby3ExpressionMammalianAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK19pA-MN
Plasmid#86973PurposeChIC; genomic mapping of chromatin proteinsDepositorInsertprotein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
ExpressionBacterialAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N GFP
Plasmid#37855DepositorInsertGFP
UseRetroviralTagsFlag and HAExpressionMammalianPromoterPKGAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-Gq-P2A-dTomato (AAV9)
Viral Prep#135635-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorTagsdTomato (physically separate, not a fusion protein)Available SinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_AktPH-mCherry
Plasmid#67301PurposeFluorescent indicator for PI(3,4,5)P3DepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWP89
Plasmid#61264PurposeIncP-based replicating vector expressing dTomato under tac promoter.DepositorInsertPtac-dTomato
UseSynthetic BiologyExpressionBacterialPromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-5p
Plasmid#103357PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-218-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
RN3P-KDM5B-FLAG
Plasmid#86398PurposeFLAG-tagged mouse KDM5B cDNA clone for mammalian cell expressionDepositorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPPromoterEF1aAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLI1_pet28a
Plasmid#131641PurposeBacterial expression of ETS gene FLI1DepositorAvailable SinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN-miRFP670nano3-WPRE-hGHpA
Plasmid#184688PurposeMammalian expression, miRFP670nano3DepositorInsertmiRFP670nano3
UseAAVPromoterhSYNAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-ProteoTuner DD
Plasmid#67181PurposeCRISPaint universal donor for fusion tagging allowing inducible destabilizationDepositorInsertProteoTuner DD
Available SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
p18S.1 wt
Plasmid#51727Purposetranscribes wild type 18S without modificationsDepositorInsert18S
Promoterpol-IAvailable SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-Akt1
Plasmid#86594PurposeExpresses TagBFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
ExpressionMammalianAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cultivarium POSSUM Toolkit
Plasmid Kit#1000000234PurposeThe POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.DepositorAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB533-42B3mutAC2-sfGFP
Plasmid#186777PurposeRNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cellsDepositorInsert42B3mutAC2-scFv
TagssfGFPExpressionMammalianAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7b-WPRE (AAV1)
Viral Prep#104489-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants, and can be used for imaging of small neuronal processes. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-Ubiquitin Fusion Vector
Plasmid#236943PurposeExpress HaloTag(R)-Ubiquitin Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertUbiquitin (UBC Human)
TagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-ACVR2A
Plasmid#23654DepositorInsertACVR2A (ACVR2A Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-kRasV12
Plasmid#114318PurposeTET-inducible expression of the V12-mutant form of the ki-Ras proteinDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV500 empty vector
Plasmid#33348DepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX
Plasmid#164611PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in mammalian cellsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
ExpressionMammalianMutationS383TPromoterCMVAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-APEX2
Plasmid#154936PurposeNLS-V5-MCP-APEX2DepositorInsertNLS-V5-MCP-APEX2
UseLentiviralTagsV5ExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-Nb37
Plasmid#205514PurposeEndosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertendo-Nb37
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2053
Plasmid#142912PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS DRR mCherry Donor EF1a BFP
Plasmid#98896PurposeHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.DepositorInsertNeomycin-T2A-mCherry-GFP
UseSynthetic BiologyExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP1830
Plasmid#70708PurposeHuman expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAGDepositorInsertmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationE782K, N968K and R1015H in SaCas9PromoterCAGAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable SinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
RNA-binding protein pooled CRISPR knockout library
Pooled Library#141438PurposeKnockout library targeting RNA-binding proteins with 10 guide RNAs per target.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only