167,601 results

1. pFUSEss-CHIg-mG3_M18

   Plasmid

   #82356

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
   

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

   Plasmid

   #21845

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Expression

   Mammalian

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-TLR9-YFP

   Plasmid

   #13642

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 9 (TLR9 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRESCA-syntide

   Plasmid

   #132415

   Purpose

   FRET-based sensor for CaMKII activity

   Depositor

   Margaret Stratton

   Article

   Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

   Insert

   FRESCA-syntide
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP1183

   Plasmid

   #104163

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TPC1-G-GECO1.2

   Plasmid

   #207143

   Purpose

   Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPCN1 (TPCN1 Human)
   

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGPN-TMPRSS2

   Plasmid

   #187484

   Purpose

   Expression vector - TMPRSS2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Promoter

   EFS

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs GFP-LC3-RFP

   Plasmid

   #117413

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Promoter

   TNNT2

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pABE8e-NG-403Q-Cterm-AAV

    Plasmid

    #194652

    Purpose

    C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG C-term
    

    Use

    AAV

    Tags

    BPNLS and NpuC split intein

    Promoter

    TNNT2

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. TPC2-G-GECO1.2

    Plasmid

    #207141

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18T-mini-Tn7T-Tp-dsRedExpress

    Plasmid

    #65036

    Purpose

    TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pEGFP-mKOR

    Plasmid

    #179285

    Purpose

    Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

    Insert

    kappa opioid receptor (Oprk1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. cytoplasmic cpVenus control

    Plasmid

    #186788

    Purpose

    Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPSF1

    Plasmid

    #155475

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF1 (CPSF1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-IDH2-WT

    Plasmid

    #87926

    Purpose

    Expresses IDH2 (wild-type) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-TRAIL

    Plasmid

    #10953

    Depositor

    Bingliang Fang

    Article

    Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

    Insert

    Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEG BacMam C term 3C eGFP His8

    Plasmid

    #160687

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    3C eGFP His8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.2-Cx43-HA

    Plasmid

    #49851

    Purpose

    Encodes human connexin 43 with a C-terminal HA epitope fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

    Plasmid

    #85428

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-12
    

    Use

    Lentiviral

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA/Myc TCF4

    Plasmid

    #16512

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBBR1k-J23107-GFPuv

    Plasmid

    #106389

    Purpose

    pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRS314-URA3

    Plasmid

    #11004

    Depositor

    Francisco Malagon

    Insert

    URA3 (URA3 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-FLEx-ER-TurboID

    Plasmid

    #160857

    Purpose

    Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Has Service

    AAV8

    Insert

    ER TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Promoter

    CAG

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3-CD68 promoter/enhancer

    Plasmid

    #34837

    Depositor

    Peter Murray

    Article

    Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

    Insert

    CD68 promoter/enhancer -680/+140 (CD68 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #179682

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GPI-RpHLuorin2

    Plasmid

    #171721

    Purpose

    GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to GPI anchor
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1_Lifeact-mCherry

    Plasmid

    #67302

    Purpose

    Fluorescent indicator for F-actin

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Lifeact-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDEST pcDNA5-FRT/TO-eGFP-Rif1

    Plasmid

    #52506

    Purpose

    mammalian expression vector of Rif1 siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    RIF1 (RIF1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: ATAGTAC

    Available Since

    June 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNO286-3

    Plasmid

    #107746

    Purpose

    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

    Depositor

    Jeffrey Tabor

    Article

    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Deleted AA 223 and 512

    Promoter

    J23106 and J23108

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR223-MAP2K1

    Plasmid

    #23406

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HRE-luciferase

    Plasmid

    #26731

    Purpose

    Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

    Depositor

    Navdeep Chandel

    Article

    Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

    Insert

    Hypoxia Responsive Elements (3)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

40. pCT5-bac2.0

    Plasmid

    #119872

    Purpose

    Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

    Depositor

    Claudia Schmidt-dannert

    Article

    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

    Inserts

    CymR
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pveg promoter with CuO sequence and PxylR promoter

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge