We narrowed to 4,855 results for: U6...

1. pMCB619

   Plasmid

   #171011

   Purpose

   CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   U6:sgRNA_Puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-C9orf72

   Plasmid

   #83439

   Purpose

   Expresses Cas9 and a gRNA targeting C9orf72

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human C9orf72 (C9orf72 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcU6_1 sgRNA

   Plasmid

   #92395

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.1 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chick U6.1

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE655

   Plasmid

   #153253

   Purpose

   M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Chicken ß-actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHDR-USR-eGFP

   Plasmid

   #208830

   Purpose

   HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

   Depositor

   Zhiying Zhang

   Article

   Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-GSK3β-#1

   Plasmid

   #32496

   Depositor

   Alex Toker

   Article

   Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

9. Circular 200,100 with interspersed loops (FANCC)

   Plasmid

   #180192

   Purpose

   AAV vector carrying a guide RNA targeting the human FANCC mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Circular 200,100 with interspersed loops (CTNNB1)

    Plasmid

    #180193

    Purpose

    AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Circular 200,100 with interspersed loops (SMAD4)

    Plasmid

    #180191

    Purpose

    AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

    Plasmid

    #198863

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human TERT gene

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    TERT sgRNA (TERT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. px459-Hira KO sgRNA

    Plasmid

    #186938

    Purpose

    Hira KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Hira KO sgRNA (Hira Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-eCas9-sgNT

    Plasmid

    #140238

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-AsCas12f1-v5.1+T1-empty

    Plasmid

    #204977

    Purpose

    AsCas12f1-v5.1+T1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nature Catalysis (2023) 6, 695–709

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N70Q/K103R/A104R/S118A/D364R

    Promoter

    PCMV,U6

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-SAG1-DHFR

    Plasmid

    #80322

    Purpose

    Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    SAG1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX552-EF1a-DIO-mcherry(with gRNA scaffold)

    Plasmid

    #199581

    Purpose

    Expresses mCherry in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    mCherry
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a intron A and U6

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6c

    Plasmid

    #117223

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6c promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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