We narrowed to 7,308 results for: RAP

1. PCR4-Hsp68::mCherry-H11

   Plasmid

   #211940

   Purpose

   dual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

   Depositor

   Evgeny Kvon

   Article

   Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSP68

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM60-S6K1(wt) barcoded

   Plasmid

   #48801

   Purpose

   expresses wt S6K1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG and barcode: GGATCC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1 RagA shRNA #2

   Plasmid

   #30320

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagA shRNA (RRAGA Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pXGFPC-5 Pxyl-dcas9 (Sth3)

   Plasmid

   #133317

   Purpose

   expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTripz Ctrl-IRES-mCherry

   Plasmid

   #187295

   Purpose

   Doxycyclin inducible expression of ctrl IRES-mCherry

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   IRES-mCherry
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_PGK_SNIPR_ADAM17 site

   Plasmid

   #188349

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_ADAM17site_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS_235

   Plasmid

   #195460

   Purpose

   Lentiviral Integratable, 4OHT/TMX-inducible expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - ZF3 - p65 - ERT2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

   Plasmid

   #211839

   Purpose

   Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   R181A R185A

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211841

   Purpose

   Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA and mCherry

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXZX351

    Plasmid

    #169454

    Purpose

    pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATα
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBMN mCherry-TAX1BP1(ΔUBD)

    Plasmid

    #119689

    Purpose

    For mammalian expression of TAX1BP1 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    UBD deletion (1-755)

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

    Plasmid

    #199356

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEG R2-L3 #BGV193

    Plasmid

    #48952

    Purpose

    For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. piCRg Entry

    Plasmid

    #58904

    Purpose

    Cas9/gRNA Entry expression plasmid

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pADH100

    Plasmid

    #90980

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6HTC_FSCN1-Halo

    Plasmid

    #175343

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    FSCN1 (FSCN1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD2529-CAG-Flag-mouse TGFb3

    Plasmid

    #180262

    Purpose

    Mouse full length TGFβ3 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    TGFβ3 (Tgfb3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH6HTC_MANF-Halo

    Plasmid

    #175341

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    MANF (MANF Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-nlsGFP-P2A

    Plasmid

    #80808

    Purpose

    nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5 HA3-eDHFR-T2A-neo

    Plasmid

    #86396

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A neo
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A neo

    Expression

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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