We narrowed to 13,608 results for: crispr cas9

1. PRE-Hsp70BbCas9_1.3

   Plasmid

   #190797

   Purpose

   aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP791_PB_dCas9-SSSavi_BirA

   Plasmid

   #211786

   Purpose

   PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pCAGG abd pEF1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRE-Hsp70BbCas9_1.2

   Plasmid

   #190796

   Purpose

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro-SMCR8

   Plasmid

   #83440

   Purpose

   Expresses Cas9 and a gRNA targeting SMCR8

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human SMCR8 (SMCR8 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. IVT-nCas9(H840A)

   Plasmid

   #207455

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   H840A
   

   Expression

   Bacterial

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR2B-dCas9-KRAB

   Plasmid

   #115547

   Purpose

   Entry cassette for dCas9-KRAB

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Insert

   dCas9-KRAB
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFREE2-eSpCas9-sgRNA

   Plasmid

   #179580

   Purpose

   Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   eSpCas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL-CRISPR.SFFV.tRFP

    Plasmid

    #57826

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

    Plasmid

    #132667

    Purpose

    Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)
    

    Expression

    Mammalian

    Promoter

    4xHRE_YB TATA

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid

    #210710

    Purpose

    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    M11

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #187341

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid

    #217800

    Purpose

    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9_YTHDF2_sgRNA

    Plasmid

    #186673

    Purpose

    YTHDF2 sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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