We narrowed to 18,199 results for: ERG

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LentiV2-hU6-evopreQ1

Plasmid

#210189

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Article

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Promoter

CAGG

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFP-VP64-GB1

Plasmid

#141417

Purpose

Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

Depositor

Izuho Hatada

Article

Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

Insert

scFvGCN4sfGFP-VP64-GB1

Tags

HA and sfGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pC018 - LshCas13a from Leptotrichia shahii

Plasmid

#91908

Purpose

Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LshCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

Plasmid

#136627

Purpose

Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-R59

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pOxyR_rfp

Plasmid

#194155

Purpose

Red Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P07

Plasmid

#124788

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a SnoopTag-MBP

Plasmid

#72323

Purpose

Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-MBP

Tags

N-terminal His6 tag. MBP is fused at the C-termin…

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRAM28

Plasmid

#234314

Purpose

pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 2

Use

Synthetic Biology

Promoter

P. CymRC

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki67-mCherry

Plasmid

#183748

Purpose

Expresses EGFP-Ki67-mCherry

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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mt POLRMT

Plasmid

#205059

Purpose

POLRMT protein expression

Depositor

Smita Patel

Article

Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

Insert

POLRMT (POLRMT Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV7 mitoDendra2

Plasmid

#157757

Purpose

Expression of the fluorescent protein Dendra2 targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

Dendra2

Expression

Mammalian

Mutation

MTS coxVIII +Dendra2

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRGE32

Plasmid

#63159

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

Depositor

Liqun Luo

Article

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

mGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z02

Plasmid

#120045

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMTS-hFluc

Plasmid

#177716

Purpose

Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

Mitochondrial Targeting Sequence MTS SS

Expression

Bacterial, Mammalian, and Y…

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGGF_RT_FAST_RFP

Plasmid

#136979

Purpose

Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

Depositor

Klaus Theres

Article

Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

Insert

seed coat marker RFP

Expression

Bacterial

Mutation

BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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EpsilonCOP EGFP

Plasmid

#128151

Purpose

Expression of epsilon COP

Depositor

Jennifer Lippincott-Schwartz

Article

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

epsilon COP (COPE Bovine)

Expression

Mammalian

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P19

Plasmid

#124789

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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