We narrowed to 13,452 results for: Mpl;

1. pYpet-Optop38i

   Plasmid

   #89749

   Purpose

   Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   Optop38i3
   

   Use

   Fluorescent protein

   Tags

   Ypet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1 FLCN (F118D) FLAG GFP

   Plasmid

   #164979

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   F118D

   Promoter

   UBC

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-HSPA5P

   Plasmid

   #44150

   Purpose

   a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

   Depositor

   Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   HSPA5 promoter (HSPA5 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEBTet-SNAP-Cep128

   Plasmid

   #136823

   Purpose

   Mammalian expression of the centrosomal protein Cep128 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep128 (CEP128 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-AviMta2-3xFiBsd

   Plasmid

   #140964

   Purpose

   full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

   Depositor

   Brian Hendrich

   Article

   Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   metastasis-associated gene family, member 2 (Mta2 Mouse)
   

   Tags

   3xFLAG, Avi, and TEV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAPM-D4 miR30-AGO2 ts1

   Plasmid

   #115853

   Purpose

   AGO2 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   AGO2 shRNA (AGO2 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TT 3xFLAG-MAVS

   Plasmid

   #158628

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF143 pAcGFP1 SOD1G37R

   Plasmid

   #26404

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G37R

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

9. iDuet101a-mCHF1

   Plasmid

   #55611

   Purpose

   Lentivirus containing mouse Hey2 coding region

   Depositor

   Michael Chin

   Article

   Hartman et al PLoS One. 2014 Mar 25;9(3):e93033. doi:

   Insert

   Hey2 (Hey2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-IP-hWIPI1A-3×FLAG

    Plasmid

    #215509

    Purpose

    Expresses FLAG tagged human WIPI1A.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-hnRNPA2_LC

    Plasmid

    #98661

    Purpose

    Expresses MBP-tagged hnRNPA2 AA 190-341

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

    Plasmid

    #33255

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and phenylalan…

    Promoter

    SV40

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. Flag Depdc5 pLJM1

    Plasmid

    #46336

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBMN mCherry-p62(W340A/ΔUBD)

    Plasmid

    #119688

    Purpose

    For mammalian expression of LIR mutant p62 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    W340A & UBD deletion (1-385)

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/NDUFS8/myc-His

    Plasmid

    #127489

    Purpose

    Expresses myc-tagged NDUFS8 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS8 (NDUFS8 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmiRGLO-miR-124-sponge (pos. CTRL)

    Plasmid

    #117327

    Purpose

    Dual luciferase assay positive control for miR-124 binding

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    miR-124 sponge
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. shTCF7L2 # 2

    Plasmid

    #42558

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pAPM-D4 miR30-AGO2 ts2

    Plasmid

    #115854

    Purpose

    AGO2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO2 shRNA (AGO2 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGS-CD64-2

    Plasmid

    #109191

    Purpose

    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    Full-length engineered CD64, derived from human CD64
    

    Tags

    Twin-Strep

    Expression

    Insect

    Mutation

    engineered for efficient expression, see publicat…

    Promoter

    Polyhedrin

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS-mTagRFP-TUBA1B

    Plasmid

    #62850

    Purpose

    Donor vector for gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    mTagRFP-TUBA1B (TUBA1B Human, Synthetic)
    

    Use

    Yeast-e.coli shuttle vector

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. qTAG-C-moxGFP-Puro-H3C2

    Plasmid

    #207783

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid

    #59826

    Purpose

    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pmiRGLO-miR-124-sponge_rev(neg. CTRL)

    Plasmid

    #117326

    Purpose

    Dual luciferase assay negative control for miR-124 binding

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    reverse miR-124 sponge
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-EGFP-C1-HsNot1iso1-V1251R_R

    Plasmid

    #147376

    Purpose

    Mammalian Expression of HsNot1iso1-V1251R

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-V1251R (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-EGFP-C1-HsNot8-D40AE42A_AH

    Plasmid

    #148902

    Purpose

    Mammalian Expression of HsNot8-D40AE42A

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsNot8-D40AE42A (CNOT8 Human)
    

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pD649-HAsp-SEMA3C-COMP5AP-AviTag-9xHis

    Plasmid

    #157525

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3C (SEMA3C Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid

    #157691

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAC8_PH-GST-Nter-GWs-Lox (VE5586)

    Plasmid

    #163766

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal GST tag
    

    Tags

    GST Tag

    Expression

    Insect

    Promoter

    PH

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEBTet-SNAP-Cep76

    Plasmid

    #136813

    Purpose

    Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep76 (CEP76 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs-puro HA-Atg14*dCC

    Plasmid

    #38266

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed A618T, A621T, C624A and T627C for siRNA r…

    Promoter

    LTR

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

33. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid

    #207768

    Purpose

    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pEBTet-SNAP-Cep41

    Plasmid

    #136794

    Purpose

    Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep41 (CEP41 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEBTet-SNAP-α-tubulin

    Plasmid

    #136791

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-TUBA (DNMBP Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-AviMta3-3xFiBsd

    Plasmid

    #140962

    Purpose

    full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

    Depositor

    Brian Hendrich

    Article

    Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

    Insert

    metastasis associated 3 (Mta3 Mouse, Synthetic)
    

    Tags

    3xFLAG, Avi, and TEV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLNHA-C1-HsNot9_18-285_S

    Plasmid

    #147480

    Purpose

    Mammalian Expression of HsNot9_18-285

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot9_18-285 (CNOT9 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT7-EGFP-C1-HsNot9_18-285_S

    Plasmid

    #147482

    Purpose

    Mammalian Expression of HsNot9_18-285

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot9_18-285 (CNOT9 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEBTet-SNAP-γ-tubulin

    Plasmid

    #136799

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-TUBG (TUBG1 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

43. CD8a-hnRNP-M-EGFP

    Plasmid

    #86054

    Purpose

    A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CD8a luminal and transmembrane domians along with…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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45. pGAPTrap-IRESMuro

    Plasmid

    #82336

    Purpose

    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    IRESMuro
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

    Plasmid

    #74263

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.R404X truncation

    Promoter

    CMV

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

47. pGEX-6P-1-INTS3-C

    Plasmid

    #128417

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    INTS3 C-terminal region (514-1042 aa)

    Promoter

    Tac promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

49. pEBTet-SNAP-Cep70

    Plasmid

    #136821

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep70 (CEP70 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCAG-MBP-Foldon-ATG9 (830-839)

    Plasmid

    #188091

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (830-839)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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