We narrowed to 7,308 results for: RAP

1. pBx1-VHH template1-3XMyc-Spacer

   Plasmid

   #176208

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template1-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Expression scaffold for dCas9+sgRNA

   Plasmid

   #171799

   Purpose

   PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Insert

   PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4XBS2WT-Luc

   Plasmid

   #16593

   Depositor

   Bert Vogelstein

   Article

   Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

   Insert

   p53 binding sites (TP53 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pVCERC-5 Pvan-dcas9 (Sth3)

   Plasmid

   #133319

   Purpose

   expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   vanillate

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH6HTC_mmHUR-Halo

   Plasmid

   #175311

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HUR (Elavl1 Mouse)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRMTK-clo6

   Plasmid

   #203957

   Purpose

   Cloning plasmid containing the kanamycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Kanamycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJBL724

   Plasmid

   #140394

   Purpose

   Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-hucO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MTK8b_004

   Plasmid

   #123892

   Purpose

   Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   5' HIV LTR (3rd gen.)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21(+)-His6-Ubc13

   Plasmid

   #212715

   Purpose

   Bacterial expression of His6-Ubc13

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pH6HTC_CCDC59-Halo

    Plasmid

    #175327

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC59 (CCDC59 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH6HTC_BTF3-Halo

    Plasmid

    #175324

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLIPglu-2uDelta13

    Plasmid

    #12990

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F D152A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    D152A mutation in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMN-018

    Plasmid

    #195422

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-PGK-CRY2PHR-VPR

    Plasmid

    #192608

    Purpose

    Lentiviral vector for constitutive expression of CRY2PHR-VPR.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    CRY2PHR-VPR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TOPO pTRE

    Plasmid

    #68457

    Purpose

    TOPO construct expressing pTRE for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Tetracycline Response Element Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE:shRNA A-pGK:rtTA-Cre

    Plasmid

    #68471

    Purpose

    Inducible lentivirus expressing an arbitrary shRNA and Cre

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    GFP-shRNA A-miR30
    rtTA-2A-Cre
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK and pTRE

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YF-Rac1(DN)

    Plasmid

    #20151

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(DN) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dominant negative mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK7_014

    Plasmid

    #123881

    Purpose

    Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3' HIV LTR (3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK3b_031

    Plasmid

    #123809

    Purpose

    Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    KRAB:meCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge