We narrowed to 15,157 results for: pET

1. pET32a-hHBEGF

   Plasmid

   #199234

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proheparin-binding EGF-like growth factor (HBEGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-NT*-HRV3CP

   Plasmid

   #162795

   Purpose

   Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

   Depositor

   Gottfried Otting

   Article

   Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

   Insert

   Human rhinovirus 14 3C protease
   

   Tags

   NT* solubility tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a.Tat(48-57)-EGFP

   Plasmid

   #165040

   Purpose

   Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   EGFP
   

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET_mNG2(1-10)_32aalinker_mNG2(11)

   Plasmid

   #82611

   Purpose

   Bacterial expression vector for split mNeonGreen2 screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
   

   Expression

   Bacterial

   Mutation

   split before mutations

   Promoter

   T7

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-pA-Hia5

   Plasmid

   #174372

   Purpose

   SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein A - hia5
   

   Tags

   6xHis and Protein A

   Expression

   Bacterial

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30b-7+-SrtA

   Plasmid

   #105602

   Purpose

   expressed 7+ sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7+ mutant
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET3a-hUb

    Plasmid

    #61937

    Purpose

    Expression of hUb in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    human ubiquitin (UBC Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 RNA Polymerase

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a(CTLA4)

    Plasmid

    #177905

    Purpose

    Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CTLA4 (aa 183-223 only) (CTLA4 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDB179-DuraPETase

    Plasmid

    #218699

    Purpose

    Encodes codon optimized DuraPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DuraPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET29b-rhMOG

    Plasmid

    #225484

    Purpose

    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

    Depositor

    John Pak

    Article

    Wu et al J Immunol Methods. 2024 Oct 13:113764. d

    Insert

    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-29 (signal peptide) and 150…

    Promoter

    T7

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET30a-GABAT

    Plasmid

    #239572

    Purpose

    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

    Depositor

    William Plaxton

    Article

    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

    Insert

    4-aminobutyrate aminotransferase (gabT E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. CCM2(FL)_pET49b

    Plasmid

    #224066

    Purpose

    Bacterial expression of full-length CCM2 fused to an N-terminal GST. HRV-3C cleavage site

    Depositor

    Ben Goult

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    ccm2 (CCM2 Human)
    

    Tags

    GST; 6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pET28a(+)/FbGH17B

    Plasmid

    #100912

    Purpose

    Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Insert

    FbGH17Bcat
    

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    not full length

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pETDuet_huCAD_ICADL (TevS)

    Plasmid

    #100098

    Purpose

    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    

    Expression

    Bacterial

    Mutation

    two caspase cleavage sites (DETD-117 and DAVD-224…

    Promoter

    T7

    Available Since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. eSrtA(4S-9) pET29b

    Plasmid

    #75146

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N98D S102C A104V A118T F122A K134R F144L I182V E1…

    Promoter

    T7

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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