We narrowed to 2,832 results for: CAG promoter

1. pCAG-AviMta2-3xFiBsd

   Plasmid

   #140964

   Purpose

   full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

   Depositor

   Brian Hendrich

   Article

   Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   metastasis-associated gene family, member 2 (Mta2 Mouse)
   

   Use

   Tags

   3xFLAG, Avi, and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. U6-hGRIN2B-CAG-ps-SaCas9

   Plasmid

   #102853

   Purpose

   A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, S. aureus, Synthetic)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter;

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-mPpp3cc-FLAG-6XHIS

   Plasmid

   #179160

   Purpose

   Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
   

   Use

   Tags

   6XHIS and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-hPhyB-mCherry-HRasCT

   Plasmid

   #100281

   Purpose

   Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa)

   Promoter

   CAG promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-FLEX-SomArchon

   Plasmid

   #126943

   Purpose

   Cre-dependent expression of SomArchon under CAG promoter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

   Insert

   SomArchon
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-DIO-tTA

   Plasmid

   #127091

   Purpose

   An AAV genome with Cre-dependent expression of tTA from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   CAG

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-SMS1-TEV-HAT

   Plasmid

   #202557

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS1 (SGMS1 Human)
   

   Use

   Tags

   Histidine-Affinity-tag (HAT) and TEV cleavable si…

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   CAG and chicken β-actin promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-CAG-empty-ires-EGFP

    Plasmid

    #122953

    Purpose

    Lentiviral expression empty vector under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS SARS-CoV-2 XBB Spike

    Plasmid

    #195287

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 XBB Spike

    Depositor

    Marceline Côté

    Insert

    SARS-CoV-2 XBB Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24-27…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    Feb. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183605

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAG-mRuby2(miRE.FF4)-TS.FF4x1-bGH

    Plasmid

    #235298

    Purpose

    ComMAND closed-loop circuit regulating mRuby2, CAG-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (synthetic cytomegalovirus early enhancer + c…

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPy-CAG-Cre::ERT2-IRES-BSD

    Plasmid

    #48760

    Purpose

    CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Cre::ERT2
    

    Use

    Ppy

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG APX W41F dimeric (mito matrix)

    Plasmid

    #40307

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APX W41F (dimeric)
    

    Use

    Tags

    Flag

    Expression

    Mutation

    W41F mutation.

    Promoter

    CAG

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCaggs-mGold2t

    Plasmid

    #231764

    Purpose

    Expression of mGold2t in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-Akt-FoxO3a-KTR-EGFP

    Plasmid

    #125131

    Purpose

    Fluorescent reporter for Akt activity

    Depositor

    Kazuhiro Aoki

    Article

    Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

    Insert

    FOXO2 (FOXO3 Human)
    

    Use

    Tags

    Enhanced green fluorescent protein (EGFP)

    Expression

    Mammalian

    Mutation

    FoxO3A 1-402 amino acid

    Promoter

    CAG promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid

    #199252

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity eSpCas9(1.1)-D10A nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid

    #127239

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-DIO(ChRger1-TS-YFP)

    Plasmid

    #127245

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger1-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CAG-DIO(ChRger3-TS-YFP)

    Plasmid

    #127242

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger3-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Use

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid

    #100281

    Purpose

    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa)

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-FLEX-SomArchon

    Plasmid

    #126943

    Purpose

    Cre-dependent expression of SomArchon under CAG promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/Puro-CAG-ASAP2s

    Plasmid

    #101274

    Purpose

    Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CAG-DIO-tTA

    Plasmid

    #127091

    Purpose

    An AAV genome with Cre-dependent expression of tTA from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CAG

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid

    #100282

    Purpose

    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-621 aa)

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV PHP.eB-CAG-Venus

    Plasmid

    #233695

    Purpose

    AAV expression of Venus from CAG promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

    Plasmid

    #231347

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)

    Plasmid

    #231349

    Purpose

    Self complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAGGS-SMS1-TEV-HAT

    Plasmid

    #202557

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Use

    Tags

    Histidine-Affinity-tag (HAT) and TEV cleavable si…

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. aav-CAG-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113761

    Purpose

    AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    CAG

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPB-CAG-Tfe3::ERT2-pgk-hph

    Plasmid

    #48756

    Purpose

    PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Tfe3::ERT2 (Tcfe3 Mouse)
    

    Use

    Piggybac

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid

    #223721

    Purpose

    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.CAG.iAChSnFR-NULL

    Plasmid

    #137956

    Purpose

    Expresses iAChSnFR (non-binding variant) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (non-binding variant)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Y140A binding site mutant

    Promoter

    CAG

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAGGS-Akt-FoxO3a-KTR-EGFP

    Plasmid

    #125131

    Purpose

    Fluorescent reporter for Akt activity

    Depositor

    Kazuhiro Aoki

    Article

    Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

    Insert

    FOXO2 (FOXO3 Human)
    

    Use

    Tags

    Enhanced green fluorescent protein (EGFP)

    Expression

    Mammalian

    Mutation

    FoxO3A 1-402 amino acid

    Promoter

    CAG promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid

    #199252

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity eSpCas9(1.1)-D10A nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid

    #127239

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-CAG-DIO(ChRger1-TS-YFP)

    Plasmid

    #127245

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger1-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-CAG-DIO(ChRger3-TS-YFP)

    Plasmid

    #127242

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger3-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. aav-CAG-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113761

    Purpose

    AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    CAG

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pPB-CAG-Tfe3::ERT2-pgk-hph

    Plasmid

    #48756

    Purpose

    PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Tfe3::ERT2 (Tcfe3 Mouse)
    

    Use

    Piggybac

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

47. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid

    #223721

    Purpose

    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid

    #221002

    Purpose

    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25-IRES-BleoR
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAAV-CAG-EGFP-P2A-FusionRed

    Plasmid

    #191108

    Purpose

    Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    pAAV-CAG-EGFP-P2A-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge