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We narrowed to 2,424 results for: dcas9

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Showing: 301 - 320 of 2424 results

  1. dCas9-FOG1[N+C]

    Plasmid
    #100085
    Purpose
    dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    FOG1(aa 1-45) (ZFPM1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-FOG1-dCas9-FOG1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100085-plasmid-map-sequence-id-197489

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  5. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139090-plasmid-map-sequence-id-273886

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  6. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Expression
    Insect
    Promoter
    act5C
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62211-plasmid-map-sequence-id-101870

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  7. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  10. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100936-plasmid-map-sequence-id-206124

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  11. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Publication
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

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  15. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid
    #100941
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
    Tags
    1xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    100941-plasmid-map-sequence-id-206116

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  17. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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Showing: 301 - 320 of 2424 results