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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 3181 - 3200 of 14352 results

  3. TRE3G-PE2-P2A-BFP

    Plasmid
    #231580
    Purpose
    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein
    Depositor
    Jesse Engreitz
    Publication
    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.
    Insert
    PE2-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    231580-plasmid-map-sequence-id-462114

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  6. pUC19-E6WZS9

    Plasmid
    #103103
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6WZS9(Cas9 coding gene from Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103103-plasmid-map-sequence-id-201812

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  7. pUC19-A8LN05

    Plasmid
    #103083
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A8LN05(Cas9 coding gene from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103083-plasmid-map-sequence-id-201724

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  8. pGinB-8X(GGS)-dCas9-FLAG-NLS

    Plasmid
    #81205
    Purpose
    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus
    Depositor
    David Liu
    Publication
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pGinB-8X(GGS)-dCas9-FLAG-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81205-plasmid-map-sequence-id-154468

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  9. p-dCas9-HDAC1-H141A-Hygro

    Plasmid
    #104417
    Purpose
    transient expression of dCas9-dHDAC1 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dHDAC1 (HDAC1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H141A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104417-plasmid-map-sequence-id-204223

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  11. pEF-XMAS-1xStop

    Plasmid
    #164411
    Purpose
    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.
    Depositor
    Xiao Wang
    Publication
    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164411-plasmid-map-sequence-id-337634

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  16. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid
    #71391
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Publication
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71391-plasmid-map-sequence-id-128452

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Showing: 3181 - 3200 of 14352 results