169,450 results
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Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialPromoterT7Available SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-gRNA-EF1a-EGFP-CAAX
Plasmid#167936PurposegRNA entry vectorDepositorInsertU6-gRNA backbone
UseLentiviralPromoterU6Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIP
Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorAvailable SinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Klotho (secreted)
Plasmid#17713DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-JNK KTR-mCherry
Plasmid#115493PurposeExpression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cellsDepositorInsertJNK KTR-mCherry
TagsmCherryExpressionMammalianMutationmCherry G229L, mCherry Y298 deletedPromoterCAGAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP
Plasmid#30130DepositorInsertEnhanced green fluorescent protein
UseLentiviralExpressionMammalianAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
Multiplex Yeast Toolkit (MYT)
Plasmid Kit#1000000229PurposeThe Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.DepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependent Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Integrin-Beta1-N-18
Plasmid#54129PurposeLocalization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509DepositorAvailable SinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0389
Plasmid#141600PurposeLentiviral vector for overexpressing the SMARCA4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE
Plasmid#179256PurposeAAV-mediated expression of GCaMP8s under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVExpressionMammalianPromoterCAGAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-1
Plasmid#129726PurposeExpressing SpCas9 and sgAAVS1-1DepositorInsertSpCas9 and sgAAVS1-1
UseCRISPRTagsP2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterTREAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-EGFP
Plasmid#50469PurposeEGFP under the control of CaMKIIa promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsN/APromoterCaMKIIaAvailable SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin_psychLight2
Plasmid#163909PurposeTo generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoterDepositorHas ServiceAAV9InsertpsychLight2
UseAAVTagsFlag tagExpressionMammalianPromoterSynapsinAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-N1
Plasmid#60561PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-G418-C4
Plasmid#160342PurposeHigh-copy expression vector for K. marxianus, G418 selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
TagsSapphireExpressionYeastPromoterHIS3Available SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ-mgU2-47-Mango II
Plasmid#127586PurposeExpresses mgU2-47-Mango II scaRNA from a mU6 promoterDepositorInsertmgU2-47 scaRNA with Mango II tag
ExpressionMammalianPromotermodified U6Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MERTK
Plasmid#23900DepositorInsertMERTK (MERTK Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-3xFLAG-V5
Plasmid#87071PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseLentiviralTags3xFLAG-V5ExpressionMammalianAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
ExpressionBacterialPromoterhsp60Available SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Gattinara in pRDA_118 backbone
Pooled Library#136986PurposeDesigned for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only