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Plasmid#189773PurposeConstruct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusionDepositorInsertmScarlet3-Giantin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1
Plasmid#54767PurposeLocalization: N1 Cloning Vector, Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-APP-mGFP
Plasmid#196704PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB3-mScarlet3_N1
Plasmid#189770PurposeConstruct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3DepositorInsertEB3-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FZ-NbPDS
Plasmid#184268PurposepAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.DepositorInsertCPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
UseTagsExpressionPlantMutationThe protease processing site between MP and L-CP …PromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)
Viral Prep#105543-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromotercTNTTagsEGFPAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f (AAV9)
Viral Prep#135632-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDi-P2A-mCherry
Plasmid#204358PurposeAAV vector for miniDi expression under the control of human synapsin promoterDepositorInsertminiDi-P2A-mCherry
UseAAVTagsmCherryExpressionMutationThe third intracellular loop (ICL3) of hM4Di was …PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX_DSB_Spectrum-V3
Plasmid#192476PurposeLentiviral DSB Spectrum V3 Reporter SystemDepositorInsertBFP1.2, mCherry, incompleteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV2)
Viral Prep#105530-AAV2PurposeReady-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Membrane protein gRNA library
Pooled Library#113345PurposeContains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAsDepositorSpeciesHomo sapiensUseLentiviralAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-EV
Plasmid#163454Purposeempty vector control for pLJC6DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA Donor_hEF1a
Plasmid#229779PurposeBxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment markerDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCE-hUL
Plasmid#41855PurposeNon-integrating (episomal) expression of human L-MYC and LIN28DepositorUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianMutationPromoterHuman cytomegalovirus immediate early enhancer/pr…Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-GFP
Plasmid#115495PurposeLentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)DepositorInserteGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1180 U6-reci Gag-pol v2
Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUdeltaGW-rtTA
Plasmid#19780Purpose3rd generation lentiviral vectorDepositorInsertreverse tetracycline transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVX_DSB_Spectrum-V1
Plasmid#192474PurposeLentiviral DSB Spectrum V1 Reporter SystemDepositorInsertBFP1.2, incompleteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_peroxisome_C1
Plasmid#85063PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-Scrambled
Plasmid#47541PurposeTet inducible scrambled shRNA.DepositorInsertScrambled shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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AAV-hSyn-Soma-jGCaMP8m (AAV9)
Viral Prep#169257-AAV9PurposeReady-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MET-V5/HIS
Plasmid#201982Purposeexpression of human MET receptor tyrosine kinase in mammalian cellsDepositorInsertMET receptor tyrosine kinase (MET Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV1)
Viral Prep#62723-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent expression of ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagstdTomato (Cre-dependent)Available sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only