We narrowed to 17,901 results for: REV
-
Plasmid#191219PurposeLentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cellsDepositorInsertSPIKE_SARS2_Delta (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationT19R 156del 157del R158G L452R T478K D614G P681R …PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA
Plasmid#191220PurposeLentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cellsDepositorInsertSPIKE_SARS2_Lambda (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationG75V T76I Del 246-252 L452Q F490S D614G T859NPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-IFIT5_K160R-VA
Plasmid#191222PurposeLentiviral expression of human IFIT5_K160R in mammalian cellsDepositorInsertinterferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
UseLentiviralTagsVA tagExpressionMammalianMutationchanged Lysine 160 to Arginine (K160R)PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-HiBiT
Plasmid#191212PurposeTransient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assayDepositorInsertSARS-CoV-2 spike_WT (S SARS CoV-2)
UseTagsHiBiT (high affinity small nanoluciferase fragmen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-p62 HRD
Plasmid#207550PurposeHomologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-P62
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag
Plasmid#231601PurposeExpress circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoterDepositorInsertTornado-1xALFATag-5xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCARGO-RMCE
Plasmid#233264PurposeBackbone plasmid used to clone lncRNA inserts in preparation for RMCEDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol
Plasmid#232976PurposeGalactose iduced expression of Gcn4 solvvol in yeastDepositorInsertGcn4 solvvol
UseTagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WHA
Plasmid#232995PurposeGalactose iduced expression of Gcn4 ILVtoED W+HAin yeastDepositorInsertGcn4 ILVtoED W+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAHA
Plasmid#232997PurposeGalactose iduced expression of Gcn4 9acidAHA in yeastDepositorInsertGcn4 9acidA
UseTags3xHA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAGFP
Plasmid#233009PurposeGalactose iduced expression of Gcn4 9acidAGFP in yeastDepositorInsertGcn4 9acidA
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WGFP
Plasmid#233007PurposeGalactose iduced expression of Gcn4 LVtoED W+GFPin yeastDepositorInsertGcn4 ILVtoED W+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol W
Plasmid#232977PurposeGalactose iduced expression of Gcn4 solvvol W+ in yeastDepositorInsertGcn4 solvvol W+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 SATtoG KtoR
Plasmid#231861PurposeBacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoRDepositorInsertGcn4 SATtoG KtoR
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-NFAPorig
Plasmid#221091PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-NFAPorig
Plasmid#221086PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-CFAPorig
Plasmid#221087PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-Strep-KDEL
Plasmid#228527PurposeLentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.DepositorInsertStrep-KDEL
UseLentiviralTagsStreptavidin-KDELExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Kan
Plasmid#232102PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif1A-EGFP-SNAP
Plasmid#229851PurposeKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif1A-EGFP-SNAP (Kif1a Rat)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-deltaCKAR
Plasmid#222429PurposeFRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.DepositorInsertPlasma membrane delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
Plasmid#231603PurposeExpress circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoterDepositorInsertTornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA
Plasmid#231604PurposeExpress circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoterDepositorInsertTornado-1xALFATag-5xSunTag_codon_optimized-8xAAA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot
Plasmid#231593PurposeExpress circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoterDepositorInsertTornado-2xALFATag-10xSunTag-Pseudoknot sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SSD1-L1374
Plasmid#226278PurposePlasmid expressing the SSD1 allele from L-1374, under control of its native promoterDepositorInsertSSD1 (SSD1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG2A HRD
Plasmid#207540PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cellsDepositorInsertHaloTag flanked by human ATG2A locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Talin-1 WT
Plasmid#201798PurposeExpresses Talin-1 protein in MAC-Tag-C backboneDepositorInsertTalin-1 (Tln1 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSKm4-ATF1
Plasmid#221133PurposeFormaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detectionDepositorInsertATF1 (ATF1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterPm4Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pAM72
Plasmid#227622PurposepETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)DepositorUseTagsHis-PrescissionExpressionBacterialMutation1-179 and aa 2-239, G77PPromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-deltaCKAR
Plasmid#222430PurposeFRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.DepositorInsertNucleus delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM315 pACYC-His6-Rpn10[ΔUIM]
Plasmid#226348PurposeExpression of yeast Rpn10 with the UIM deletedDepositorInsertrpn10 (RPN10 Budding Yeast)
UseTagsHisExpressionBacterialMutationdelta UIMPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Golgi-deltaCKAR
Plasmid#222428PurposeFRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.DepositorInsertGolgi delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS315-SEC22-NCYC110
Plasmid#226273PurposePlasmid expressing the SEC22 allele from NCYC110, under control of its native promoterDepositorInsertSEC22 (SEC22 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS315-SEC22-L1374
Plasmid#226271PurposePlasmid expressing the SEC22 allele from L-1374, under control of its native promoterDepositorInsertSEC22 (SEC22 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS315-SEC22-UWOPS872421
Plasmid#226272PurposePlasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoterDepositorInsertSEC22 (SEC22 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS315-SEC22-S288C
Plasmid#226270PurposePlasmid expressing the SEC22 allele from S288C, under control of its native promoterDepositorInsertSEC22 (SEC22 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML107-PMR1
Plasmid#226264PurposePlasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 geneDepositorInsertPMR1 gRNA (PMR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SSD1-NCYC110
Plasmid#226279PurposePlasmid expressing the SSD1 allele from NCYC110, under control of its native promoterDepositorInsertSSD1 (SSD1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgSeLEU2
Plasmid#224870PurposeDisruption of S. eubayanus type LEU2 genesDepositorInsertpYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUG-IRES-mCherry
Plasmid#222109PurposeStart codon reporter (WT AUG)DepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1559
Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-coSnaA-TEV-His_His-TEV-SnaC
Plasmid#225059Purposerecombinant expression of SnaA in E. coliDepositorInsertSnaA
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ar2
Plasmid#225220PurposeExpression androgen receptor 2 in mammalian cellsDepositorInsertandrogen receptor 2
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
300_pETcon_SARS2_FLip
Plasmid#222230Purposeyeast surface display of the SARS-CoV-2 FLip variant RBDDepositorInsertSARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
297_pETcon_SARS2_Omicron-BA286
Plasmid#222231Purposeyeast surface display of the SARS-CoV-2 BA.2.86 variant RBDDepositorInsertSARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
299_pETcon_SARS2_EG-5
Plasmid#222232Purposeyeast surface display of the SARS-CoV-2 EG.5 variant RBDDepositorInsertSARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only