167,605 results

1. Cbh_v5 AAV-CBE N-terminal

   Plasmid

   #137175

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE N-terminal; U6-protospacer
   

   Use

   AAV

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-miRFP670nano3

   Plasmid

   #184664

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIP-GFP-IRF3

   Plasmid

   #127663

   Purpose

   Over-express EGFP-IRF3

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   IRF3 (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 mCherry

   Plasmid

   #128073

   Purpose

   puro exchanged to mCherry

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   no

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. B2380_BTB-IDR

   Plasmid

   #215098

   Purpose

   Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR) in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   BTB-mCherry-FUS(IDR)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. tetO-HAND2

   Plasmid

   #170690

   Purpose

   doxycycline-inducible overexpression of HAND2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   HAND2 (HAND2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER-SPLICSs-cFAST10

   Plasmid

   #210431

   Purpose

   Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   ER-SPLICSs-cFAST10
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

   Plasmid

   #209857

   Purpose

   Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

   Depositor

   Tim Brown

   Inserts

   IL2-ss
   VHH (G08 variant)
   Rabbit Fc Gamma 1
   

   Tags

   x3 MycTag and x6 HisTag

   Expression

   Mammalian

   Promoter

   hEF1-HTLV promoter

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_NRL_P2A_Hygro_Barcode

    Plasmid

    #120469

    Purpose

    Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NRL (NRL Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

    Plasmid

    #126049

    Purpose

    For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

    Viral Prep

    #112677-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTTP1B

    Plasmid

    #91723

    Purpose

    integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA

    Depositor

    Graham Hatfull

    Article

    Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23

    Type

    Empty backbone

    Use

    Integration-proficient plasmid vectors for mycoba…

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-GATA3

    Plasmid

    #116747

    Purpose

    Lentiviral expression of GATA3

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    GATA3 (GATA3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

    Plasmid

    #126552

    Purpose

    Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    CD8a and GFP

    Mutation

    Delta gag/pol

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-IP-HaloTag7-NCOA4

    Plasmid

    #192806

    Purpose

    Stable expression of HaloTag7-NCOA4 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLSL.R.Ly

    Plasmid

    #227697

    Purpose

    Reengineered geminiviral replicon vector

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EPS15-GFP-His

    Plasmid

    #170860

    Purpose

    Mammalian expression of EPS15-GFP-His.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Tags

    EE and GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLYS1-FLAG-MitoGFP-HA

    Plasmid

    #50057

    Purpose

    Expression of GFP targeted to mitochondria with Flag and HA tags

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    Mito-GFP
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

    Plasmid

    #111259

    Purpose

    mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA-HA-ER WT

    Plasmid

    #49498

    Purpose

    Expresses HA-tagged ER wild-type in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. G555

    Plasmid

    #216483

    Purpose

    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

    Depositor

    Christophe Danelon

    Insert

    plsB, plsC, cdsA, pssA
    

    Use

    Cell-free expression

    Promoter

    T7 for each gene

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1(-) MID49 4xMyc Hisx6

    Plasmid

    #44596

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD49 (Mief2 Mouse)
    

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP_IRES_tdTomato

    Plasmid

    #184046

    Purpose

    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

    Depositor

    Angela Pannier

    Article

    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

    Inserts

    Enhanced Green Fluorescent Protein
    tdTomato
    Internal Ribosome Entry Site
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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