We narrowed to 15,064 results for: pET

1. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMVHA E2F1

   Plasmid

   #24225

   Purpose

   Mammalian expression of human E2F1 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGal4BD-CRY2

   Plasmid

   #28243

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX_DSB_Spectrum-V2

   Plasmid

   #192475

   Purpose

   Lentiviral DSB Spectrum V2 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRY2PHR-mCherryN1

   Plasmid

   #26866

   Purpose

   Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2PHR (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-N1-MITF-M

   Plasmid

   #38131

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   MITF-M (MITF Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. G1333 DddAtox-C–dSpCas9

   Plasmid

   #157834

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DR274

   Plasmid

   #42250

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Worm

   Mutation

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCB092 FoMV-sgMgCh

   Plasmid

   #234815

   Purpose

   To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting SbMgCh
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. K44A dynamin 1 pEGFP

    Plasmid

    #34681

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGal4BD-CRY2PHR

    Plasmid

    #28244

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    cry2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRY2FL(deltaNLS)-mCherryN1

    Plasmid

    #26871

    Purpose

    Mammalian expression of cytoplasmic CRY2-mCherry

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NLS sequences deleted

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHL 1405

    Plasmid

    #53010

    Purpose

    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains -56 to +141 relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. siRNA-resistant SigmaR1-mCherry

    Plasmid

    #226567

    Purpose

    Encodes siRNA resistant SigmaR1 protein labeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mNeonGreen-Lrrc59

    Plasmid

    #226564

    Purpose

    Encodes Lrrc59 protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sg2.02 16x(MS2) MUC4.1

    Plasmid

    #101154

    Purpose

    gRNA with 16 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCGN-HCF-1 fl

    Plasmid

    #53309

    Purpose

    plasmid contains human HCFC1 full length gene with HA and c-myc tags

    Depositor

    Winship Herr

    Article

    Wilson et al Cell. 1993 Jul 16;74(1):115-25.

    Insert

    HCFC1 (HCFC1 Human)
    

    Use

    Tags

    HA and c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-Lrrc59

    Plasmid

    #226565

    Purpose

    Encodes Lrrc59 protein laebeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCA528-PPxY1-GS-WW3e

    Plasmid

    #166705

    Purpose

    expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT PPxY1 fused to NEDD4L WW3
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. siRNA-resistant SigmaR1-mNeonGreen R119A

    Plasmid

    #226572

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. siRNA-resistant SigmaR1-mNeonGreen D188A

    Plasmid

    #226573

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    D188A

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. siRNA-resistant SigmaR1-mNeonGreen F191A

    Plasmid

    #226571

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    F191A

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Use

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-N1-MITF-D

    Plasmid

    #38133

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-D (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZac2.1-GfaABC1D-GAT3-HA-mCherry

    Plasmid

    #117722

    Purpose

    Expresses a GABA transporter GAT-3 (Slc6a11)

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Slc6a11 (Slc6a11 Mouse)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.hSyn.flex.H2B.GFP

    Plasmid

    #170368

    Purpose

    Cre-inducible AAV vector expressing nuclear GFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    flex H2B GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCB036 FoMV-sgGFP

    Plasmid

    #234813

    Purpose

    To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid

    #172841

    Purpose

    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-EF1a-4mtGCaMP6f-DIO

    Plasmid

    #179529

    Purpose

    Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Insert

    4mtGCaMP6f
    

    Use

    AAV

    Tags

    4x Cox8 targeting sequence

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC57-Amp_CRISPIE donor B1

    Plasmid

    #172842

    Purpose

    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTO149_RFP_CauNEO

    Plasmid

    #177277

    Purpose

    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    RFP, CauNEO
    

    Use

    Tags

    ENO1-RFP

    Expression

    Bacterial

    Mutation

    All CUG Leucine codons were replaced with the syn…

    Promoter

    AgTEF2

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLKO mouse shRNA 1 rictor

    Plasmid

    #21341

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    rictor shRNA 1 (Rictor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-S in pAR9 (rep/cap)

    Plasmid

    #174539

    Purpose

    encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

    Depositor

    Casey Maguire

    Article

    Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

    Insert

    VP1 of AAV9 containing the AAV-S 7-mer peptide insert
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHAGE-EFS-PCP-3xSNAP-NLS

    Plasmid

    #236388

    Purpose

    Overexpression of PCP-3xSNAP in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    PCP-3xSNAP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. gfp-Arkadia/RNF111

    Plasmid

    #112228

    Purpose

    Expresses GFP-tagged Arkadia in mammalian cells

    Depositor

    Vasso Episkopou

    Article

    Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

    Insert

    (Arkadia)Rnf111 (Rnf111 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDL-hu FceRI alpha

    Plasmid

    #8365

    Depositor

    Jean Kinet

    Article

    Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

    Insert

    human Fc epsilon RI alpha cDNA (FCER1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

42. gWiz_BM40-vGAT-VL-hIgG1-CL

    Plasmid

    #218437

    Purpose

    mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-VL-hIgG1-CL
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MMLV-RT(dRH) for Split-PE (pEH50)

    Plasmid

    #190106

    Purpose

    Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT(delta RNAseH)
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pPB-CAG-ECC-Biosensor

    Plasmid

    #140832

    Purpose

    Encoding the CAG promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    ECC-Biosensor
    

    Use

    Piggybac (pb) transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCMVHA E2F2

    Plasmid

    #24226

    Purpose

    Mammalian expression of human E2F2 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F2 (E2F2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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49. NFAST(low)-mApple-mito

    Plasmid

    #214415

    Purpose

    Mitochondria localization of low-affinity NFAST fused to mApple-mito

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-mito

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA3.1-FLAG-SBP-STAG2 (WT)

    Plasmid

    #73963

    Purpose

    STAG2 cDNA (CCDS43990) wild type

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only