163,347 results

1. MET-HYGRO

   Plasmid

   #218113

   Purpose

   Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmGFP-P2A-K(AAA)20-P2A-RFP

   Plasmid

   #105688

   Purpose

   Double fluorescent stalling reporter K(AAA)20

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K(AAA)20
   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIRES-EGFP-puro

   Plasmid

   #45567

   Depositor

   Michael McVoy

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   IRES-EGFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Phcmv (strong human cytomegalovirus immediate ear…

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CA

   Plasmid

   #69616

   Purpose

   To express gene strongly with AAV

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

   Type

   Empty backbone

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNPK-NLS-Vika

   Plasmid

   #79969

   Purpose

   expresses human codon-optimized Vika-recombinase in mammalian cells

   Depositor

   Frank Buchholz

   Article

   Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

   Insert

   human codon-optimized Vika sequence, NLS tagged
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPEE13

   Plasmid

   #128359

   Purpose

   Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Insert

   Acetamidase (amdS)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF5/FRT/DEST-3xHA/mGli2

   Plasmid

   #51250

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

9. TFORF2910

   Plasmid

   #143665

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIC (NFIC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF2912

    Plasmid

    #143667

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIC (NFIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Hyg-mEGFP

    Plasmid

    #191847

    Purpose

    Expresses the monomeric form of EGFP

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRSV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPR220.08

    Plasmid

    #78553

    Purpose

    Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

    Depositor

    Jeffrey Tabor

    Article

    Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

    Insert

    uirS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    J23108

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_CRY1_WT

    Plasmid

    #82264

    Purpose

    Gateway Donor vector containing CRY1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CRY1 (CRY1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA, EGFP Spastin M87 with exon 4

    Plasmid

    #167542

    Purpose

    For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

    Depositor

    Tarun Kapoor

    Article

    Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

    Insert

    Spastin M87 with exon 4 (SPAST Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWSK29

    Plasmid

    #172972

    Purpose

    Low copy number cloning vector

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-BRD2

    Plasmid

    #65376

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD2 (BRD2 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETSXM2-pLacI-mtrC

    Plasmid

    #174615

    Purpose

    Bacterial expression of mtrC from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    MtrC
    

    Use

    Synthetic Biology; Shuttle vector in e. coli and …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGRNA2e

    Plasmid

    #169782

    Purpose

    Insert one gRNA

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGRNA1

    Plasmid

    #169778

    Purpose

    Insert one gRNA

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR221-SLC39A12_STOP

    Plasmid

    #161295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A12 (SLC39A12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid

    #51248

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907,980,1006 mutated to A…

    Promoter

    EF1a

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pBS-3xpA

    Plasmid

    #176505

    Purpose

    3x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    3x SV40 polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR221-SLC30A5_STOP

    Plasmid

    #161243

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A5 (SLC30A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pDONR221-SLC30A6_STOP

    Plasmid

    #161231

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A6 (SLC30A6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Use

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs-Sox2

    Plasmid

    #13367

    Purpose

    Retroviral expression of mouse Sox2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    SRY-box containing gene 2 (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUG-Pten

    Plasmid

    #140732

    Purpose

    Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-Pten (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. qTAG-C-mStayGold-Blast

    Plasmid

    #227254

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF2883

    Plasmid

    #144255

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BHLHA9 (BHLHA9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pECO200

    Plasmid

    #169765

    Purpose

    Express SpCas9 and Basta resistance gene

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Plant binary vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S, AtU6

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQCXIB-H2B-mCherry

    Plasmid

    #164244

    Purpose

    retroviral plasmid for H2B-mCherry

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    H2B
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

    Plasmid

    #51251

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848,923,956 mutated to Ala

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pSCM001

    Plasmid

    #124605

    Purpose

    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

    Depositor

    David Summers

    Article

    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

    Insert

    mCherry-pHluorin
    

    Use

    Tags

    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

    Expression

    Bacterial

    Mutation

    Promoter

    pDAB

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPR219.05

    Plasmid

    #78554

    Purpose

    Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output

    Depositor

    Jeffrey Tabor

    Article

    Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

    Insert

    uirR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    J23115

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDONR221-SLC30A4_STOP

    Plasmid

    #161255

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A4 (SLC30A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. mEmerald-Fidgetin-C-18

    Plasmid

    #62756

    Purpose

    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Fidgetin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

    Plasmid

    #44070

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge