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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 321 - 340 of 3270 results

  1. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62886-plasmid-map-sequence-id-105909

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  2. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Promoter
    xylose
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133317-plasmid-map-sequence-id-266835

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  4. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74215-plasmid-map-sequence-id-139305

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  5. DS-NMcas

    Plasmid
    #48646
    Purpose
    Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    NM tracrRNA
    Use
    CRISPR
    Promoter
    NM tracrRNA promoter and proC
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48646-plasmid-map-sequence-id-71690

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  6. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  9. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid
    #133319
    Purpose
    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Promoter
    vanillate
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133319-plasmid-map-sequence-id-266830

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  12. pCAG Cas9-2A-Citrine

    Plasmid
    #92358
    Purpose
    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 2A Citrine
    Expression
    Mammalian
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92358-plasmid-map-sequence-id-178229

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  14. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100902-plasmid-map-sequence-id-195323

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  16. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100903-plasmid-map-sequence-id-196607

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Showing: 321 - 340 of 3270 results