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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of TagBFP-Rab5 fusion

Depositor

Gia Voeltz

Article

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM3D(Gq)

Plasmid

#45547

Purpose

Expresses hM3D (Gq) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM3D (Gq)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Article

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
RSV-Sp1

Plasmid

#12098

Depositor

Robert Tjian

Insert

Sp1 (SP1 Human)

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Article

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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DsRed-rab9 WT

Plasmid

#12677

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Article

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCSDest

Plasmid

#22423

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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EB3-tdTomato

Plasmid

#50708

Purpose

expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

Depositor

Erik Dent

Article

Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

Insert

EB3 (MAPRE3 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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