We narrowed to 87,741 results for: lias
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Plasmid#213221PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR4 (CXCR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-iSeroSnFR
Plasmid#128484PurposeFluorescent reporter for serotonin (mammalian expression, membrane localization tag)DepositorInsertiSeroSnFR
UseTagsIg-kappa leader, Myc, PDGFR + enhanced ER export,…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
CCR6-DuET
Plasmid#213202PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR6 (CCR6 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P
Plasmid#179458PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA-
Plasmid#229698PurposeTransient expression of GFP-alpha-cateninA- in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationL785A, I792A, V796APromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-WPRE
Plasmid#179460PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorInsertMS2-P65-HSF1 (HSF1 Synthetic, Human)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)
Plasmid#162880PurposeExpression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFPDepositorInsertPhosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A2-Neo
Plasmid#189743PurposeThe construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A2 (ALDH1A2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCE-KdGl
Plasmid#154880PurposeEpisomally expresses KDM4D and GLIS1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninΔβH
Plasmid#229705PurposeTransient expression of GFP-alpha-catenin-delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA+
Plasmid#229697PurposeTransient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin bindingDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA+ΔβH
Plasmid#229704PurposeTransient expression of GFP-alpha-cateninA+deltabetaH in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninWT
Plasmid#229709PurposeTransient expression of GFP-alpha-cateninWT in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.16-RBD
Plasmid#213070PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variantDepositorInsertpCAGGS/XBB.1.16-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomaGCaMP7
Plasmid#182942PurposeCre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-Omicron-BA.5
Plasmid#213828PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variantDepositorInsertpαH-S-GSAS/Omicron-2P-BA5 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-DsRed(isoform 2)
Plasmid#99563Purposemammalian expression of TMEM230-dsRed (isoform 2)DepositorInsertTMEM230 (TMEM230 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCE-K2N
Plasmid#154879PurposeEpisomally expresses KLF2 and NANOG in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLViN-iRFP670-α-cateninWT
Plasmid#229702PurposeLentiviral expression of iRFP670-alpha-cateninWT in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCC1-4k-BBclone2
Plasmid#137070PurposeE. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1DepositorInsertsBB0323
P13
DipA
Lmp1
UseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SomaGCaMP7
Plasmid#182941PurposeCre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
elavl3:SomaGCaMP7f
Plasmid#158760PurposeCell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseSynthetic BiologyTagsExpressionMutationPromoterHuC promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
Plasmid#64219PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2ADepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFP-P2A-E1BExpressionMammalianMutationPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLViN-iRFP670-α-cateninA+
Plasmid#229703PurposeLentiviral expression of iRFP670-alpha-cateninA+ in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Soma-jGCaMP8f
Plasmid#182943PurposeCell body-targeted jGCaMP8f, under synapsin promoter.DepositorInsertSoma-jGCaMP8f
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 HxD
Plasmid#136365PurposeExpresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2xStrepTagII, FLAG, and HAExpressionMammalianMutationHxD (H1077Y, D1079A)PromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P-Kv
Plasmid#179462PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P-Kv
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187960PurposeAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CAMK2D-T287A
Plasmid#221689PurposeMammalian expression of FLAG-tagged human CAMK2D (T287A)DepositorInsertCAMK2D (CAMK2D Human)
UseTagsExpressionMammalianMutationT287APromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only