Addgene

We narrowed to 29,586 results for: REP

Showing: 3401 - 3420 of 29586 results

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pDendra2-H3

Plasmid

#157814

Purpose

Fluorescent reporter for histone H3 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H3 (H3c1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Use

Tags

Lamp1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-TTF-1-wt

Plasmid

#74990

Purpose

Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 (TTF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

wild-type, full-length, without 3' UTR

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

NLS (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
ACTIN-SOX2-PGK Cre

Plasmid

#59019

Purpose

Bicistronic Lentivirus expressing sox2 and Cre

Depositor

Trudy Oliver

Article

Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

B-actin

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCytERM-Electra1

Plasmid

#179476

Purpose

ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-Electra1

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMK277 (mClover-NeoR)

Plasmid

#72793

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Neo

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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ALC1-c007

Plasmid

#173112

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Use

Tags

Nanoluc

Expression

Bacterial

Mutation

L613-K879, codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMA_Int

Plasmid

#110096

Purpose

E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Insert

mycophage integrase

Use

Required for transformation of attb integrating v…

Tags

Expression

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pActin-Electra1

Plasmid

#184941

Purpose

beta-actin labelling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-beta-Actin

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt-Electra1-P2A-mScarlet

Plasmid

#196113

Purpose

zebrafish expression of nbt-Electra1-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-Electra1-P2A-mScarlet

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CamK2-tTA

Plasmid

#210731

Purpose

AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

Depositor

Wei Xu

Article

Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

tetracycline-controlled transactivator (tTA)

Use

AAV

Tags

Expression

Mutation

Promoter

CamK2

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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gRNAU6

Plasmid

#135963

Purpose

Contains tracrRNA-hU6 sequence for double sgRNA cloning

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1_d77

Plasmid

#172588

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

deleted amino acids 1-76

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG-IRES2-mNeonGreen

Plasmid

#175506

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G-mCMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2b

Plasmid

#157817

Purpose

Fluorescent reporter for histone H2B tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

Histone H2B (H2bc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6020nes

Plasmid

#108656

Purpose

A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-JNK-EV

Use

Tags

XFP

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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