204,515 results
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Plasmid#132569PurposeDonor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 alleleDepositorInsertHuman MYH11 knockin homology arms (MYH11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ADAM10-HA
Plasmid#65106PurposeExpression of HA tagged ADAM10 in mammalian cellsDepositorInsertADAM10 (ADAM10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2115
Plasmid#49140PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSBbi-RH
Plasmid#60516PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV2)
Viral Prep#26969-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEYFPAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pProEx Rac1(G12C) CL
Plasmid#224269PurposeProtein purification of Rac1 (G12C)DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionBacterialMutationG12CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CD36-C-10
Plasmid#54030PurposeLocalization: Membrane, Excitation: 487, Emission: 509DepositorInsertCD36 (CD36 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCap-101-Apr
Plasmid#227507PurposeCapture plasmid with apramycin resistance and pIJ101 repliconDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pComb8
Plasmid#63889PurposeFor phage display of antibody libraries by fusion to modified geneVIII.DepositorTypeEmpty backboneUsePhage displayTagsgeneVIIIExpressionBacterialMutationPromoterlacZAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-tdTomato
Plasmid#44332DepositorHas ServiceAAV5InserttdTomato
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4_10xUAS-MLPmin-luc2
Plasmid#194383PurposeReporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoterDepositorInsert10xUAS-luc2
UseTagsExpressionMammalianMutationPromoterMLP minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-Cas9-2A-Puro
Plasmid#172855PurposeIVT template plasmid for making modified Cas9Puro RNADepositorInsertCas9-2A-Puro
UseIvt template plasmidTags2A-PuroExpressionMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH2B-mNG2(11)
Plasmid#206043PurposePlasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.DepositorInsertH2B gene fused to the mNG2(11) fragment
UseSynthetic BiologyTagsH2B-mNG2(11)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac PlayBack
Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Antibody#218107-rAbPurposeAnti-V5 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence.DepositorRecommended ApplicationsWestern BlotReactivitySource SpeciesRabbitIsotypeIgG1Trial SizeNot available to purchaseAvailable sinceMay 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pBK-CMV-hNHERF2
Plasmid#47801PurposeExpresses human NHERF2 in mammalian cellsDepositorInsertNHERF2 (NHERF2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-ND1-AD
Plasmid#183900PurposeExpresses TALED_Right-ND1-AD in mammalian cellsDepositorInsertTALED for ND1 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD4_mCherry
Plasmid#187386PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRTDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-HA-gate-pGK-HYG
Plasmid#107396PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sasgRNACMV- mCherry-WPREpA
Plasmid#217015PurposeS. aureus sgRNAs backbone along with mCherry expressionDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF.CMV.RFP
Plasmid#17619DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-R1441C
Plasmid#25046DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationArginine 1441 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits