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We narrowed to 59,428 results for: cloning

Showing: 3481 - 3500 of 59428 results

  2. LA802: pMVP (L3-L2) AID + WPRE

    Plasmid
    #121794
    Purpose
    pMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectors
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    AID + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121794-plasmid-map-sequence-id-237296

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  6. pMOD_B2000

    Plasmid
    #91059
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    none (begins with P2A to be fused to TALEN_1)
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91059-plasmid-map-sequence-id-181870

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  7. pMOD_B2403

    Plasmid
    #91071
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91071-plasmid-map-sequence-id-181880

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  8. PC2328

    Plasmid
    #232089
    Purpose
    Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.
    Depositor
    Pranam Chatterjee
    Article
    Hong et al Nat Commun. 2025 Apr 15;16(1):3555. doi:
    Insert
    duAb with cloning Esp3I restriction site
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232089-plasmid-map-sequence-id-469903

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  9. pSG068

    Plasmid
    #214259
    Purpose
    pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    Expression
    Bacterial
    Mutation
    18 nucleotide 5' of the start codon (Shine-D…
    Promoter
    araBAD promoter
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214259-plasmid-map-sequence-id-432475

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  10. pSG059

    Plasmid
    #214262
    Purpose
    PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
    Tags
    6x His-tag and 6xHis-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214262-plasmid-map-sequence-id-432470

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  11. pCOA-Leu-FvPPT1

    Plasmid
    #179923
    Purpose
    Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Insert
    FvPPT1
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179923-plasmid-map-sequence-id-359550

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Showing: 3481 - 3500 of 59428 results