We narrowed to 25,959 results for: Vit;

1. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Akt2(T309A)

   Plasmid

   #86625

   Purpose

   Expresses eGFP-tagged human Akt2(T309A) phosphomutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(T309A) (AKT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

   Plasmid

   #74101

   Purpose

   vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-mCherry-N1-iChloC

   Plasmid

   #85467

   Purpose

   improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Insert

   iChloC
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, T159C, D156N

   Promoter

   CMV (+enhancer)

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MS2-adRNA (5'UAG)

   Plasmid

   #170126

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(5'UAG)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEG R1-ChlorR-R4 #BGV049

   Plasmid

   #48943

   Purpose

   Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. BoxB-MS2 adRNA (RAB7A-UAG site)

   Plasmid

   #170138

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(UAG)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-puro-TTF-1-HDD

   Plasmid

   #74991

   Purpose

   Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   TTF-1 HDD mutant (NKX2-1 Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   HDD = homeodomain deleted

   Promoter

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPSP6

   Plasmid

   #48150

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-sh-mSox10-2

    Plasmid

    #37007

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSox10-2 (Sox10 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pHAGE-eGFP-OPTN E50K

    Plasmid

    #175765

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E50K

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-Teton-puro-shFAK #1

    Plasmid

    #198757

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #1 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-Teton-puro-shFAK #2

    Plasmid

    #198758

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #2 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCASP-SptP120-TD-HilA

    Plasmid

    #153330

    Purpose

    Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIND-HBSP

    Plasmid

    #53113

    Purpose

    Expression of GAL4BD-tagged Hepatitis B splicing protein

    Depositor

    Xu Lin

    Article

    Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

    Insert

    HBSP
    

    Use

    Tags

    GAL4BD

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Mef2D-shRNA

    Plasmid

    #32900

    Depositor

    Michael Greenberg

    Article

    Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

    Insert

    Mef2d (Mef2d Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1-RNA

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. MCP-ADAR2-DD(E488Q, N496F)

    Plasmid

    #170139

    Purpose

    AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-ADAR2-DD(E488Q, N496F)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-eGFP-TAX1BP1 SKICH (1-140D)

    Plasmid

    #175768

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    SKICH (1-140D)

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. IBMc071

    Plasmid

    #161943

    Purpose

    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pREG(R1-R3) #BGV141

    Plasmid

    #48958

    Purpose

    Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pBabe-puro-Flag-TRAF5

    Plasmid

    #102679

    Purpose

    retroviral expression plasmid for TRAF5

    Depositor

    Hasam Habelhah

    Article

    Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

    Insert

    TRAF5 (Traf5 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hRREB1-RFP

    Plasmid

    #22924

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-Bmp15

    Plasmid

    #163594

    Purpose

    Expresses BMP-15 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp15 (Bmp15 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCASP-SptP167-3G124-HilA

    Plasmid

    #153322

    Purpose

    Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP167-3G124 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-hSyn-Gαi*-BERKY1

    Plasmid

    #158427

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR

    Plasmid

    #48157

    Purpose

    Transgenically expresses firefly luciferase protein in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    luciferase
    

    Use

    Luciferase; T. cruzi expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. D44_Ld_180090

    Plasmid

    #135858

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    alpha glucosidase II subunit, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CP1041-ABE8e-dimer

    Plasmid

    #138499

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFLAG-4EHP-mISG15

    Plasmid

    #17355

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    4EHP-mISG15 (EIF4E2 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-3xflagGREB1b

    Plasmid

    #112222

    Purpose

    mammalian expression of flag tagged GREB1b

    Depositor

    Craig Burd

    Article

    Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

    Insert

    GREB1b (GREB1 Human)
    

    Use

    Tags

    3x flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCASP-SptP120-3m160-HilA

    Plasmid

    #153325

    Purpose

    Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3m160 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. attB-P7-attP-YFP

    Plasmid

    #195571

    Purpose

    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P7

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. vsv-CenpB 1-166-Sgo1-GFP

    Plasmid

    #108495

    Purpose

    expression of vsv-CenpB-1-166-Sgo1-EGFP

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    CenpB-Shugoshin 1 (SGO1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CenpB 1-166 fusion before Sgo1

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid

    #48992

    Purpose

    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Inserts

    cre recombinase
    Luciferase
    

    Use

    Luciferase; Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pM116: CasRx VEGFA presgRNA

    Plasmid

    #166868

    Purpose

    U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-Gdf7

    Plasmid

    #163597

    Purpose

    Expresses GDF-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf7 (Gdf7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Some Guanin and Cytosine were substituted to redu…

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTRCHisB-TRF2_M

    Plasmid

    #50490

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Myb (TERF2 Human)
    

    Use

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 438-500

    Promoter

    pTrc

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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