We narrowed to 18,153 results for: Met;

1. pPD290 ZF43x6-Compact_EYFP

   Plasmid

   #138901

   Purpose

   EYFP reporter vector for the ZF43x6-Compact promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF43x6-Compact

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dKatushka-pBAD

   Plasmid

   #54775

   Purpose

   Localization: Protein Expression Vector , Excitation: 588, Emission: 635

   Depositor

   Dmitriy Chudakov, Michael Davidson

   Article

   Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

   Type

   Empty backbone

   Use

   Tags

   6xHis and dKatushka

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. CMV-CitroffRH

   Plasmid

   #158546

   Purpose

   Mammalian expression of nonbinding variant of inverse-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   CitroffRH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-RLuc-T2A-PuroR

   Plasmid

   #102320

   Purpose

   expression of r-luciferase

   Depositor

   Rudolf Jaenisch

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ubc

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pN3-mTagBFP-pHluorin-CAAX

   Plasmid

   #178345

   Purpose

   Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   mTagBFP-pHluorin-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6p-1-FRB-PafA

   Plasmid

   #113404

   Purpose

   Expresses FRB-PafA in bacteria

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   FRB-PafA
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPD934 ZF37x6-Compact_EYFP

   Plasmid

   #138929

   Purpose

   EYFP reporter vector for the ZF37x6-Compact promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF37x6-Compact
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF37x6-Compact

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

   Plasmid

   #48297

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mTB3-Myc tag

    Plasmid

    #210205

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    Myc-tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H2B-LSSmGFP

    Plasmid

    #186539

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Human Histone 2B (D26G and V119I mutations)

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSF11-wmiRFP713-T2A-HO1

    Plasmid

    #197246

    Purpose

    Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP713-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCIS.MTRIA-AVP

    Plasmid

    #184600

    Purpose

    For expression of a fluorescent sensor for vasopressin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-AVP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

    Plasmid

    #55216

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe-Hygro-GFP

    Plasmid

    #61215

    Purpose

    Retrovirus expressing GFP

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-miRFP680

    Plasmid

    #197203

    Purpose

    Expresses the protein of miRFP680 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP680
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACYCDuet_WTDHFR_WTTS

    Plasmid

    #91226

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JD94

    Plasmid

    #229479

    Purpose

    CMV promoter driving expression of adenoviral E2A gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JD80

    Plasmid

    #229480

    Purpose

    Adenoviral VA RNA I gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    VA RNA I
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPD292 ZF43x8-Compact_EYFP

    Plasmid

    #138903

    Purpose

    EYFP reporter vector for the ZF43x8-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x8-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x8-Compact

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVH233-Tier1-PmPGK1-eYFP

    Plasmid

    #169522

    Purpose

    Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-5X1 Flag PA1

    Plasmid

    #15560

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PA1 (PAGR1 Human)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

    Plasmid

    #137994

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGS_2paCRY2

    Plasmid

    #129651

    Purpose

    2P-activatable CRY2

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. negMaLionB

    Plasmid

    #113911

    Purpose

    negative control for Blue ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHis17_ParM _2

    Plasmid

    #78202

    Purpose

    Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

    Insert

    parM (K33A D63C T174A T175N D224C C287A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A D63C T174A T175N D224C C287A

    Promoter

    unknown

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

    Plasmid

    #175530

    Purpose

    Expression of HaloTag7 in the endoplasmid reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag7-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3.0_mitoNanobody

    Plasmid

    #136622

    Purpose

    Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-EnhancerNb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-VARNAM

    Plasmid

    #115552

    Purpose

    Red fluorescent voltage indicator for mammalian expression

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

    Insert

    VARNAM
    

    Use

    Tags

    Golgi and ER export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMito.iGABASnFR.F102Y.Y137L

    Plasmid

    #112177

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F102Y.Y137L

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-R-Kras

    Plasmid

    #180572

    Purpose

    ddRFP sensor of Kras

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Kras
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. zbFGF

    Plasmid

    #12309

    Depositor

    James Thomson

    Article

    Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

    Insert

    Fgf2 (fgf2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala 11 mutated to Pro, Ser 55 mutated to Asn

    Promoter

    Available since

    Sept. 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. YEp181-CUP1-His-Smt3

    Plasmid

    #99536

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUPD2+pEstrInd

    Plasmid

    #170880

    Purpose

    Phytobrick - Estradiol Inducible Promoter

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    pEstradiol_Inducible
    

    Use

    Tags

    Expression

    Plant

    Mutation

    c.837C>T and c.1353G>A to remove BsaI and E…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. C125-E01: stuffer

    Plasmid

    #162900

    Purpose

    Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mini-donor - NKX6.1-nEGFP

    Plasmid

    #206047

    Purpose

    this mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mEos2-H2B-6

    Plasmid

    #57384

    Purpose

    Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. GST-PRMT9 WT

    Plasmid

    #67607

    Purpose

    bacterial expression of wild type human PRMT9

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    tac

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Bxb1-surrogate-reporter

    Plasmid

    #65624

    Purpose

    Fluorescent reporter to monitor the activty of Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attP
    

    Use

    Bxb1

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge